T-2毒素在马铃薯品种对镰刀菌干腐病抗性评价中的应用

Application of T-2 Toxin in Evaluation of Potato Varieties Resistance to Fusarium Dry Rot

为明确T-2毒素作为抗病筛选指标的可行性,采用T-2毒素对不同抗性的马铃薯品种进行抗病性评价,对其进行抗病生理生化特性研究。结果表明,50 ng/mL T-2毒素可以作为马铃薯抗干腐病抗病性筛选的最佳指标。在生理生化测定中,经T-2毒素处理的3个品种块茎内的可溶性糖和还原糖含量均增加;细胞防御酶——超氧化物歧化酶(SOD)和过氧化物酶(POD)活性增强;苯丙氨酸代谢途径关键酶苯丙氨酸解氨酶(PAL)和4-香豆酰-辅酶A连接酶(4CL)活性提高;丙二醛(MDA)含量升高。抗病品种可溶性糖、还原糖、SOD、POD、PAL、4CL生理指标均高于感病品种,而抗病品种的MDA含量低于感病品种。由此表明,T-2毒素可以作为马铃薯抗病筛选的指标。

In order to clarify the feasibility of T-2 toxin as an index for screening the resistance of dry rot in potato,T-2 toxin was used to evaluate the resistance of potato varieties.At the same time,the physiological and biochemical indexes related to disease resistance in potato were also studied.The results showed that 50 ng/mL T-2 toxin was the best indicator for screening the dry rot resistance in potato.In the physiological and biochemical tests under the treatment of T-2 toxin the contents of soluble sugar,reducing sugar,the activities of superoxide dismutase(SOD)and peroxidase(POD),phenylalanine ammonia lyasein(PAL,a key enzyme of phenylalanine metabolism pathway),4-coumaroyl CoA ligase(4CL)and the content of malondialdehyde(MDA)increased in the tubers of three potato varieties,the contents of soluble sugar,reducing sugar and the activities of SOD,POD,PAL,4CL in resistant varieties were both higher than susceptible varieties,while the content of MDA showed the opposite trend.In conclusion,T-2 toxin can be an index for screening the resistance of dry rot in potato.