具有抗菌功能的聚乳酸基纳米纤维膜的构建及生物学评价

Construction and Biological Evaluation of Polylactic Acid-based Nanofibrous Membranes with Antibacterial Properties

材料表界面改性技术是实现材料性能可控制备,赋予其功能的关键。该研究采用喷纺技术制备了以聚乳酸(PLA)为基体的纳米纺丝可吸收高分子生物膜,并通过等离子体处理技术引入氨基,经过戊二醛将单宁酸接枝至纤维表面,有效提高了PLA膜的亲水性能。利用单宁酸的还原功能将纳米银原位沉积到纳米纤维膜表面,有效提高了纤维膜的抗菌性能。体外细胞学评估表明该材料具有良好的细胞相容性,有望作为抗菌功能可吸收生物膜用于抗感染皮肤创面修复。

The interface modification technology of material surface is crucial for realizing the controllable preparation of material properties and endowing its biological functions.In this study,a biodegradable polylactide(PLA)-based nanofibrous biofilm was prepared by an air-brushing approach,and amino groups were introduced through plasma processing technology,followed by grafting tannic acid onto the fiber surface in the presence of glutaraldehyde,which effectively improved the hydrophilicity of the modified biofilm.Furthermore,the reducibility of tannic acid allowed for in situ deposition of silver nanoparticles on the film surface,which effectively improved the antibacterial capacity of the biofilm.In vitro biological evaluation with fibroblasts as a kind of model cells showed that the material had good cytocompatibility.The functional biofilm is expected to act as a kind of self-antibacterial biodegradable film for skin wound healing.