圈养林麝粪样类固醇激素水平与麝香分泌的关系

Relationships between fecal steroid hormones and musk secretion in captive forest musk deer

基于非损伤取样和放射免疫法(RIA),于2018年9月1日—10月15日期间对四川马尔康林麝繁育场的36头雄性圈养林麝(Moschus berezovskii)的粪样类固醇激素水平进行了检测,结合麝香分泌数据采集,探究了林麝的粪样睾酮和皮质醇水平与其麝香分泌的关系,结果表明:四川马尔康圈养林麝的麝香分泌(12.53±0.76) g (n=36)与粪样睾酮水平(106.03±12.34) ng/g (n=36)呈显著正相关(r=0.436,P<0.01),泌香较多雄麝(16.50±0.61) g (n=17)的睾酮水平(141.51±21.86) ng/g (n=17)显著地高于泌香较少(8.98±0.59) g (n=19)雄麝(74.27±7.93) ng/g (n=19)(t=-3.252,df=34,P<0.01),方程y=0.021x+10.270(R2=0.120,P<0.05)可较好地拟合二者间关系。麝香分泌与皮质醇浓度(47.49±1.53) ng/g (n=36)相关不显著(r=0.078,P>0.05),泌香较多雄麝的皮质醇浓度(48.98±2.28) ng/g (n=17)略高于泌香较少雄麝(46.17±2.14) ng/g (n=19),差异不显著(P>0.05),方程y=0.038x+10.730(R2=0.023,P>0.05)可近似拟合皮质醇与泌香量间的关系。此外,雄麝睾酮水平对泌香量有显著影响(P<0.05),睾酮水平较高林麝泌香量(14.97±1.17) g (n=12)显著高于睾酮水平较低个体的泌香量(11.32±0.90) g (n=24)(F=4.79,P<0.05),而睾酮、皮质醇和年龄对麝香分泌的综合效应及变量间的交互作用均不显著(P>0.05)。研究确定了圈养林麝粪样睾酮和皮质醇水平与麝香分泌量间的关系,可用于麝类驯养实践的麝香产量预测及品系选育。

Fecal steroid hormone of 36 male captive forest musk deers(Moschus Berezovskii) in Maerkang Musk Deer Farm(MMDF), Sichuan Province was measured based on non-invasive sampling and radio immunoassay(RIA) methods from September 1st to October 15th, 2018. In combination with data of musk production, the relationships between fecal testosterone, cortisol and musk secretion were analyzed. The results showed that, musk secretion(12.53±0.76) g(n=36) was significantly positively correlated with fecal testosterone(106.03±12.34) ng/g(n=36) in MMDF′s captive forest musk deer. The testosterone concentration(141.51±21.86) ng/g(n=17) of male musk deer with over-averaged musk(16.50±0.61) g(n=17) was significantly higher than that(74.27±7.93) ng/g(n=19) of with below-averaged musk(8.98±0.59) g(n=19)(t=-3.252, df=34, P<0.01), and the equation y=0.021x+10.270(R2=0.120, P<0.05) could fit the relationship well. No significant correlation between musk secretion and the cortisol concentration(47.49±1.53) ng/g(n=36)(r=0.078, P>0.05), and cortisol concentration(48.98±2.28) ng/g(n=17) of male musk deer with over-averaged musk was slightly higher than that of with below-averaged musk(46.17±2.14) ng/g(n=19), but not significant(P>0.05). The equation y=0.038x+10.730(R2=0.023, P>0.05) approximately fitted the relationship between cortisol concentration and musk secretion. In addition, testosterone concentration significantly affected the musk secretion(P<0.05), and musk secretion(14.97±1.17) g(n=12) with higher testosterone concentration was significantly more than that(11.32±0.90) g(n=24) of with lower testosterone level(F=4.79, P<0.05), while the cohort effects of testosterone, cortisol and age on musk secretion were not significant, neither were the interactions. The definite relationships between testosterone, cortisol and musk secretion can be used in musk production prediction and strain selection.