钨矿工人HSP27与矽肺的相关性研究

Association between heat-shock protein 27 and silicosis miners

目的本文通过研究HSP27与矽肺的相关性,寻找钨矿工人患矽肺的敏感指标,以期早期筛查出钨矿工人易患矽肺的人群。方法采用病例对照研究方法,选择赣南医学院第一附属医院呼吸内科及赣州于都铁山垅镇卫生院就诊或体检的钨矿矽肺组为矽肺组55人,有钨矿工作史且胸片正常的对照组42人为对照组。所有对象于清晨空腹时抽取外周静脉血5ml,用酶联免疫吸附试验(ELISA法)检测血清HSP27水平,并进行统计分析。结果对照组的血清HSP27浓度明显高于矽肺组(P<0.05),矽肺组中不同矽肺分期血清HSP27浓度差异无统计学意义,采用二元Logistic回归分析发现,结果表明经调整年龄、工龄、吸烟混杂因素后血清HSP27水平对矽肺患病呈负相关,血清HSP27水平降低发生矽肺的风险可能增加(调整OR:0.98,95%CI:0.96~1.00)(P<0.05)。血清HSP27浓度大于42.49ng/ml的钨矿工人发生矽肺的风险是浓度小于11.05ng/ml的0.33倍(调整OR:0.33,95%CI:0.11~0.96)(P<0.05)。结论钨矿对照组血清HSP27水平显著高于矽肺组,血清HSP27水平对矽肺患病呈负相关,钨矿工人血清HSP27水平降低可能增加罹患矽肺病的风险。

Objective The aim of this study is to searched for the sensitive indicators of silicosis in tungsten miners by research Association between heat-shock protein 27 and silicosis miners,so as to screen out the population who susceptible to silicosis in tungsten miners at early stage.Methods Using the method of case-control studies,55 Tungsten mine silicosis patient were selected as the case group and the healthy workers with a history of tungsten were used as a control group.All the subjects were selected the Department of Respiratory Medicine of First Affiliated Hospital Of Gannan Medical University and Yudu Tieshan Town Health Center in Ganzhou City for a medcal or physical examination.All subjects received 5 ml of peripheral venous blood in the early morning on an empty stomach.serum HSP27 levels were detected by enzyme-linked immunosorbent assay(ELISA)(Part 2).Finally,analyze the data with statistics methed.Results The serum HSP27 concentration of healthy workers in Gannan tungsten mine was significantly higher than that of silicosis patients(P<0.05).There was no significant difference in serum HSP27 concentration between different silicosis stage in silicosis patients(P>0.05).Binary logistic regression analysis showed that serum HSP27 levels had a negative effect on the incidence of silicosis after adjusting the age,length of service and smoking confounding factors,Decreased serum HSP27 may increase the risk of silicosis(adjusted OR:0.98,95%CI:0.96-1.00)(P<0.05).The risk of silicosis in workers with serum HSP27 concentrations greater than 42.49ng/ml is 0.33 times the concentration of less than 11.05ng/ml.(adjusted OR:0.33,95%CI:0.11-0.96)(P<0.05).Conclusion The serum HSP27 level of healthy workers in Gannan tungsten mine was significantly higher than that of silicosis patients.Serum HSP27 level was significantly negatively correlated with silicosis.The decrease of serum HSP27 level in tungsten workers may increase the risk of silicosis.