不同产地斜叶檀挥发油成分的比较分析研究

Comparative Study on Volatile Oil Components in Dalbergia Pinnata(Lour.)Prain From Different Producing Areas

目的对不同产地斜叶檀挥发油成分进行比较分析,为不同产地斜叶檀的鉴定提供依据。方法采用水蒸气蒸馏法提取斜叶檀挥发油;采用GC-MS对不同产地斜叶檀挥发油成分进行分析;利用SIMCA-P软件进行主成分分析和正交偏最小二乘法判别分析。结果海南组挥发油提取率在0.29%~2.63%,云南组为0.50%~3.09%,老挝组为0.40%~7.38%,缅甸组为0.40%~4.16%;经GC-MS分析得出斜叶檀挥发油主要组分54个。通过SIMCA-P软件分析,得出海南、云南、缅甸及老挝斜叶檀挥发油共有成分11个,海南特有成分3个,缅甸特有成分3个,老挝特有成分4个,其他差异成分17个。结论不同产地斜叶檀挥发油成分存在差异,可利用此差异结合GC-MS对斜叶檀的产地进行鉴定。

OBJECTIVE To provide basis for identification of Dalbergia pinnata(Lour.)Prain,comparing difference of volatile oil composition of Dalbergia pinnata(Lour.)Prain in different producing areas.METHODS The volatile oil of Dalbergia pinnata(Lour.)Prain was extracted by steam distillation,the chemical constituents were analyzed by GC-MS.Principal component analysis and orthogonal partial least square discriminant analysis were performed by SIMCA-P software.RESULTS The extraction rate of volatile oil was 0.29%-2.63%in Hainan group,0.50%-3.09%in Yunnan group,0.40%-7.38%in Laos group and 0.40%-4.16%in Myanmar group.According to the analysis of GC-MS,54 main components of volatile oil of Dalbergia pinnata(Lour.)Prain were obtained.Through the analysis by SIMCA-P,it was concluded that there were 11 common components in the volatile oil of Hainan,Yunnan,Myanmar and Laos,3 unique components in Hainan,3 unique components in Myanmar,4 unique components in Laos,and 17 other differential components.CONCLUSION There are differences in the volatile oil components of Dalbergia pinnata(Lour.)Prain in different producing areas.This difference can be used to identify the producing areas of Dalbergia pinnata(Lour.)Prain.