养血安胎方对复发性流产血栓前状态子宫内膜容受性的改善作用研究

Study on the Effect of Yangxue Antai Recipe on Improving Endometrial Receptivity in Pre-thrombotic State of Recurrent Abortion

目的探讨养血安胎方对复发性流产(RSA)血栓前状态子宫内膜容受性的作用机制。方法对30例复发性流产患者应用养血安胎方治疗3个月经周期,治疗前后分别使用B超进行排卵监测,在黄体期采用ELLSA法检测子宫内膜组织血小板衍生因子-AA(PDGF-AA)、血管内皮生长因子(VEGF)、组织纤溶酶原激活物(t-PA)及纤溶酶原激活剂抑制剂-1(PAI-1)含量,并使用电镜及HE染色方法观察子宫内膜形态。结果经养血安胎方3个月经周期治疗后,患者血清抗凝血酶Ⅲ活性(AT-Ⅲ)、蛋白S活性(PS)较治疗前显著升高(P<0.05);子宫内膜胞饮突表达丰富及表达中等的例数较治疗前显著增加(P<0.05);子宫内膜组织PDGF-AA、t-PA含量治疗后较治疗前显著降低(P<0.05)。结论养血安胎方通过补肾活血、养血法能够改善子宫内膜的血液循环及血流灌注,提高子宫内膜的容受性。

Objective To explore the mechanism of Yangxue Antai Recipe on endometrial receptivity in pre-thrombotic state of recurrent spontaneous abortion(RSA).Methods Thirty patients with recurrent spontaneous abortion were treated with Yangxue Antai Recipe for three menstrual cycles.B-ultrasound was used to monitor ovulation before and after treatment.ELLSA method was used to detect the level of PDGF,VEGF,t-PA and PAI-1 in endometrial tissue during luteal phase.Electron microscopy and HE staining method was used to observe endometrial morphology.Results After the treatment of 3 menstrual cycles with Yangxue Antai Recipe,the patients′serum antithrombin III activity(AT-III)and protein S activity(PS)were significantly higher than before treatment(P<0.05);the endometrium The number of cases with abundant and moderate expression of pinocytosis increased significantly compared with that before treatment(P<0.05);the contents of PDGF-AA and t-PA in endometrial tissue were significantly lower after treatment than before treatment(P<0.05).Conclusion Yangxue Antai Decoction can improve the blood circulation and blood perfusion of the endometrium and improve the receptivity of the endometrium by invigorating the kidney and promoting blood circulation and nourishing blood.