枸杞木虱ORCO基因及其编码蛋白的生物信息学分析

Bioinformatics Analysis of ORCO Gene and Its Coding Protein of Poratrioza sinica

枸杞木虱是枸杞的重要害虫之一,利用生物信息学软件对该虫非典型气味受体(ORCO)基因进行分析,可为进一步研究验证枸杞木虱ORCO基因的嗅觉相关功能提供理论基础。对枸杞木虱ORCO基因及其编码蛋白进行理化性质、序列特性、结构预测、进化关系等生物信息学分析。结果表明:枸杞木虱ORCO基因编码450个氨基酸,相对分子质量51.25 KD,等电点为6.32;亚细胞主要定位于其他细胞器,不属于分泌蛋白,不存在信号肽;有7个跨膜结构,Pfam:7tm6结构域和Pfam:7tm7结构域各1个;平均疏水性为0.254,属于疏水性蛋白;编码蛋白的二级结构中,α螺旋、直链延伸、β转角、无规则卷曲分别占54%、17.78%、3.78%和24.44%,三级结构主要由α螺旋和无规则卷曲缠绕折叠形成;进化树结果表明,枸杞木虱的ORCO基因与半翅目蚜科昆虫聚为一支。本研究的枸杞木虱ORCO基因生物信息学分析结果,为研究验证该基因及其编码蛋白在枸杞木虱取食、交配和产卵等过程中所发挥的作用提供了理论基础。

Paratrioza sinica is one of the important pests of Lycium chinense.Using bioinformatics software to analyze the olfactory receptor coreceptor(ORCO)gene can provide a theoretical basis for further research and verification of olfactory function of ORCO gene of L.chinense.The physicochemical property,sequence characteristics,structure prediction and evolutionary relationship of ORCO gene of this insect and its coding protein were analyzed by bioinformatics software.The results showed that ORCO gene encoded 450 amino acids,the relative molecular mass was 51.25 KD,and the isoelectric point was 6.32.The subcellular of ORCO gene mainly located in other organelles.The ORCO protein was not the secretory protein and had no signal sequence,but it had seven transmembrane structures,two structural domains of Pfam:7 tm6 and Pfam:7 tm7.The average hydrophobicity was 0.254,so the protein encoded by this gene was a hydrophobic protein.The secondary structure of ORCO protein consisted of 54%alpha helix,17.78%extended strand,3.78%beta turn and 24.44%random coils.Meanwhile,the tertiary structure of ORCO protein was mainly formed by the winding of alpha helix and random coil.The phylogenetic tree results indicated that the ORCO gene in P.sinica was clustered with Hemiptera Aphididae.The results of this study provided a theoretical basis for the research to verify the role of the gene and its coded protein in the process of feeding,mating and spawning laying of P.sinica.