25%嗪草酮·高效氟吡甲禾灵·砜嘧磺隆可分散油悬浮剂高效液相色谱分析

Analysis of Metribuzin·Haloxyfop-P·Rimsulfuron 25% OD by HPLC

[目的]建立一种对25%嗪草酮·高效氟吡甲禾灵·砜嘧磺隆可分散油悬浮剂进行分离和测定的高效液相色谱分析方法。[方法]采用ZORBAX SB-C18反相柱,以甲醇和水(0.1%磷酸水溶液)为流动相,流速为1.0 mL/min,在254 nm条件下对试样中的嗪草酮、高效氟吡甲禾灵和砜嘧磺隆同时进行定量分析。[结果]经分析方法验证,嗪草酮、高效氟吡甲禾灵和砜嘧磺隆的线性相关系数分别为0.9995、0.9999、1,标准偏差分别为0.057、0.033、0.005,变异系数分别为0.26%、0.64%、0.32%,平均回收率分别为100.2%、99.7%、100.5%。[结论]该方法简便、快速、分离效果好,适用于复配制剂中嗪草酮、高效氟吡甲禾灵和砜嘧磺隆的定量分析。

[Aims] This study aims to establish an analytical method for separation and quantitative analysis of metribuzin·haloxyfop-P·rimsulfuron 25% OD by HPLC. [Methods] The simultaneous determination of metribuzin,haloxyfop-P and rimsulfuron was conducted with ZORBAX SB-C18 reversed phase column as fix phase, the mixture of methanol and water(0.1% phosphoric acid aqueous solution) was used as mobile phase, the flow rate was 1.0 mL/min,with UV-absorbance detector at 254 nm. [Results] The analytical methods showed that the linear correlation coefficients of metribuzin, haloxyfop-P and rimsulfuron were 0.9995, 0.9999 and 1, the standard deviations were 0.057,0.033 and 0.005, the coefficients of variation were 0.26, 0.64 and 0.32%, and the average recoveries were 100.2, 99.7 and 100.5%, respectively. [Conclusions] This method has the advantages of simple, rapid, and good separation effect,which is suitable for quantitative analysis of metribuzin, haloxyfop-P and rimsulfuron in mixture formulation.