稻田杂草萤蔺对苄嘧磺隆的抗药性机理

The Resistance Mechanism of Paddy Field Weed Scirpus juncoides Roxb. to Bensulfuron-methyl

[目的]明确辽宁省萤蔺种群对苄嘧磺隆的抗药性水平和机理。[方法]采用整株剂量反应测定抗性指数和马拉硫磷增效作用,PCR技术和效液相色谱串联质谱(HPLC-MS/MS)法测定各种群萤蔺植株对苄嘧磺隆的抗药性机理。[结果]采自水田的9个萤蔺种群对苄嘧磺隆表现出了不同水平的抗药性;WT-2、WT-6和WT-22种群的抗药性源于ALS基因突变,ALS1上的Pro-197-Ser。而代谢增强是WT-5和WT-12种群非靶标抗性的关键原因。[结论]萤蔺对苄嘧磺隆的抗药性已普遍存在;ALS靶标基因突变和代谢作用增强是萤蔺对苄嘧磺隆抗药性产生的主要原因。

[Aims] This study aims to clarify the resistance level and mechanism to bensulfuron-methyl in Scirpus juncoides Roxb. [Methods] The resistance index and the synergistic effect of malathion were determined by whole plant dose response, and the resistance mechanism of various populations of S. juncoides Roxb. to bensulfuron-methyl was determined by PCR and HPLC-MS/MS. [Results] Nine populations of S. juncoides Roxb. were collected from paddy fields showed different levels of resistance to bensulfuron-methyl, the resistance of WT-2, WT-6 and WT-22 populations resulted from the mutation of ALS gene, Pro-197-Ser on ALS1. The enhanced metabolism is the key cause of non-target resistance in WT-5 and WT-12 populations. [Conclusions] The resistance of S. juncoides Roxb. to bensulfuron-methyl has been widespread, and the resistance can be caused by ALS target gene mutation or enhanced metabolism.