丽江乌头根中的二萜生物碱成分研究

Diterpenoid alkaloids from the roots of Aconitum forrestii Stapf

为了寻找丽江乌头中结构新颖的化合物,丰富丽江乌头的化学成分多样性,本文对丽江乌头(Aconitum forrestii Stapf)根部的化学成分进行研究,采用硅胶柱层析从其乙醇提取物中分离得到14个化合物。通过HR-ESI-MS、1D和2D NMR等波谱技术鉴定了它们的结构,其中化合物1是一个新的乌头碱型二萜生物碱,命名为8-O-methyl-14-O-anisoylchasmanine(1)。其余13个化合物均为已知化合物:14-acetoxy-8-O-methylsachaconitine(2)、14-acetyltalatizamine(3)、talatisamine(4)、chasmanine(5)、ezochasmanine(6)、crassicautine(7)、geniculatine C(8)、cammaconine(9)、vilmoraconitine(10)、aconitramine A(11)、vilmorrianine G(12)、hemsleyaconitine G(13)和heterophylloidine(14)。测试了所有化合物对阿霉素诱导的H9c2心肌细胞损伤的保护活性,结果显示化合物3、10、11和12表现出一定的保护作用。

To find new compounds in Aconitum forrestii Stapf and enrich the diversity of chemical composition.A new diterpenoid alkaloid along with thirteen known diterpenoid alkaloids were isolated from the ethanol extract of the roots of A.forrestii by silica gel column chromatography.Their structures were identified as 8-O-methyl-14-O-anisoylchasmanine(1),14-acetoxy-8-O-methylsachaconitine(2),14-acetyltalatizamine(3),talatisamine(4),chasmanine(5),ezochasmanine(6),crassicautine(7),geniculatine C(8),cammaconine(9),vilmoraconitine(10),aconitramine A(11),vilmorrianine G(12),hemsleyaconitine G(13)and heterophylloidine(14)by means of spectroscopic methods such as HR-ESI-MS,1D and 2D NMR.The protective activity against adriamycin induced H9c2 myocardial cell injury of all compounds have been investigated,and the results showed that compounds 3,10,11 and 12 had a certain protective effect.