重楼皂苷Ⅶ抑制肺癌H460细胞增殖和迁移能力研究

Polyphyllin Ⅶ inhibited the proliferation and migration of lung cancer H460 cells

为研究重楼皂苷Ⅶ(polyphyllin Ⅶ)抑制人肺癌H460细胞增殖、迁移能力和诱导凋亡的作用和机制。本实验采用MTT法检测重楼皂苷Ⅶ处理后H460细胞生长抑制率,Hoechst 33258染色观察细胞形态,细胞集落形成实验考察细胞的增殖能力,划痕实验和Transwell小室实验研究H460细胞迁移和侵袭能力的改变,并通过western blot法检测在重楼皂苷Ⅶ处理前后细胞蛋白表达变化情况。结果发现,重楼皂苷Ⅶ可显著抑制H460细胞增殖,影响其集落形成,并使细胞形态发生变化,抑制细胞体外的迁移和侵袭能力,并可诱导凋亡的发生。重楼皂苷Ⅶ处理后,H460细胞中基质金属蛋白酶MMP-2和MMP-9显著降低,凋亡相关蛋白剪切型caspase-3、Bax表达增加,ICAD、Bcl-2表达降低,提示重楼皂苷Ⅶ体外可能通过调节基质金属蛋白酶抑制H460细胞迁移和侵袭,同时诱导凋亡的发生。

To study the inhibitory effects and mechanisms of polyphyllin Ⅶ on the proliferation and migration of human lung cancer H460 cells.MTT assay was used to detect the cell viability of H460 cells after treatment with polyphyllin Ⅶ.Cell morphology changes were observed by Hoechst 33258 staining.Cell colony formation assay was used to investigate the ability of cell proliferation ability.Cell migration and invasion ability were detected by wound scratch assay and Transwell chamber experiment.The expressions of proteins were detected by western blot analysis.The results showed that polyphyllin Ⅶ significantly inhibited the proliferation and colony formation of H460 cells, also inhibited cell migration and invasion in vitro,and finally induced apoptosis in H460 cells.After treatment with polyphyllin Ⅶ,MMP-2 and MMP-9 were significantly decreased in H460 cells.The expressions of apoptosis-associated proteins, such as cleaved caspase-3 and Bax were increased, in addition, ICAD and Bcl-2 were decreased, suggested that polyphyllin Ⅶ inhibited the migration and invasion of H460 cells by regulating matrix metalloproteinases, and finally induced apoptosis.