不同盐度、温度及光照对漂浮浒苔生理生态的影响

Research on the Effects of Different Salinity,Temperature and Illumination for Enteromorpha prolifera Physiological Ecology

为研究不同盐度、温度及光照对漂浮浒苔生理生态的影响,我们将实验室内培养的浒苔置于不同盐度、不同温度及不同光照强度条件下培养,测定藻体光合参数。不同盐度条件下,对浒苔叶绿素荧光参数测定结果表明,在盐度为0的条件下,浒苔样品实际量子效率Fv/Fm快速降低,随后Fv/Fm维持在较低水平,在盐度10‰~40‰范围内,在前5天各处理样品Fv/Fm没有明显变化,之后样品Fv/Fm有不同程度的降低。不同实验温度条件下,对浒苔叶绿素荧光参数测定结果表明,在5℃~25℃,浒苔Fv/Fm呈现先上升后下降趋势。不同光照条件下,对浒苔叶绿素荧光参数Fv/Fm进行测定,其数值都在第一天发生了显著降低,之后稍有波动。藻体Fv/Fm变化有所不同,处理的前2天中,所有条件下样品都有相同幅度的降低,处理第三天时,光强400μmol/(m^(2)·s)下比其他光强下的要显著低14%~16%;到培养后期,则以160μmol/(m^(2)·s)下要明显较高,即160μmol/(m^(2)·s)下藻体Fv/Fm的降低总体较为平缓。光照实验表明,浒苔在低光照条件下的实际量子效率要高于高光照条件,且最大量子效率的测定也进一步证实了该结果,表明高光照条件会引起浒苔光合作用能力下降。

In order to study the effects of salinity,temperature and light on the floating algae physiology and ecology,Enteromorpha prolifera was laboratory cultured under different salinity,temperature and light intensity to algal photosynthetic parameters.The results showed that:in different salinity conditions,the determination results of Enteromorpha prolifera on chlorophyll fluorescence parameters showed that,when the salinity was 0,Enteromorpha prolifera sample actual quantum efficiency(Yield)value decreased rapidly,then it remained at a low level,when the salinity was from 10to 40,in the former 5dtreated samples,Yield value did not change obviously,but then the sample′s value had different degrees of reduction.At different temperatures,the determination results showed that Enteromorpha prolifera on chlorophyll fluorescence parameters,when the temperature was between 5and 25,the Yield value of samples increased gradually with the temperature increasing,then with the further increase of temperature the Yield value decreased rapidly.Under different illumination conditions,the chlorophyll fluorescence parameters of Enteromorpha prolifera Yield were measured,the value in the 1Dwas significantly decreased,then had a slight fluctuation.The algae Fv/Fm changed differently,during the former 2D,all the samples were decreased equally under the same conditions,the treatment of 3D,when the intensity was 400μmol/(m^(2)·s),light intensity had significantly lower 14%-16%than before;Till the later stage of culture,the light was obviously higher than before when it was 160μmol/(m^(2)·s),so the decrease of Fv/Fm was generally flat under this condition.The illumination experiments showed that,the actual quantum efficiency of Enteromorpha proliferain low light conditions was higher than in high light,and the determination of the maximum quantum efficiency also further confirmed the results,high light condition caused the decline of Enteromorpha prolifera′s light efficiency.