生殖支原体感染对不育男性精液质量的影响

Influence of mycoplasma genitalium infection on semen quality in male infertility

目的回顾性分析不育男性感染生殖支原体(MG)与精液质量检测的结果,以探讨感染MG对不育男性精液质量的影响。方法选取2017年7月至2019年7月在重庆市妇幼保健院生殖与遗传研究所就诊的383例确诊为不育的男性患者作为研究对象。根据MG感染情况分为感染组(n=54)与未感染组(n=329)。使用核酸扩增试验检测精液中MG RNA;根据《WHO人类精液检查与处理实验室手册(第5版)》操作,检测精液量、精子浓度、精子总数、前向运动精子百分率(PR)、非前向运动精子百分率(NR)、精子总活力及精子形态;使用流式细胞仪检测精子DNA碎片指数(DFI)与精子高DNA可染性指数(HDS)。结果感染组PR及精子总活力低于未感染组,差异具有统计学意义(P<0.05);两组精液量、精子浓度、精子总数、NR比较,差异无统计学意义(P>0.05)。两组正常形态精子百分率、头部缺陷精子百分率、颈部及中段缺陷精子百分率、尾部缺陷精子百分率、畸形精子指数(TZI)及精子畸形指数(SDI)比较,差异无统计学意义(P>0.05);两组DFI及HDS比较,差异无统计学意义(P>0.05)。结论感染MG会显著降低不育男性的精子活力,对其他精液参数无明显影响。

Objective To retrospectively investigate the influence of mycoplasma genitalium(MG)infection on semen quality of male infertile.Methods 383 male infertility patients diagnosed in the Reproductive and Genetic Institute of Chongqing Health Center for Women and Children from July 2017 to July 2019 were selected as the subjects,and divided into infected group(n=54)and uninfected group(n=329)according to MG infection.Mg RNA in semen was detected by nucleic acid amplification test.The semen volume,sperm concentration,total sperm count,PR,NR,total motility and morphology of sperm were measured according to the WHO Laboratory Manual for Human Semen Examination and Treatment(5th Edition).Sperm DNA fragmentation index(DFI)and sperm high DNA stain index(HDS)were determined by flow cytometry.Results PR and sperm motility in the infected group were lower than those in the uninfected group,and the difference was statistically significant(P<0.05).There were no significant differences in semen volume,sperm concentration,total sperm count and NR between the two groups(P>0.05).There was no significant difference in the percentage of normal sperm,head sperm,neck and middle sperm,tail sperm,TZI and SDI between the two groups(P>0.05).There was no significant difference in DFI and HDS between the two groups(P>0.05).Conclusions Mycoplasma genitalium infection significantly reduced sperm motility in infertile men,but had no significant effect on other semen parameters.