脂联素在乳腺癌组织中的表达及其临床意义

The Expression of Adiponectin in Breast Cancer Tissues and Its Clinical Significance

目的研究脂联素在乳腺癌组织中的表达及与临床病理特征的关系。方法基于癌症基因组图谱(TCGA)中的乳腺癌数据,结合AmiGO2衍生的血管生成基因集,筛选出与乳腺癌血管生成有关的因子脂联素,利用GEPIA2数据库对癌症基因组图谱数据库进行分析,同时结合2019年11月~2020年8月我院手术切除且病理检查确诊为乳腺癌的癌组织及癌旁组织80例,通过免疫组织化学S-P法、qRT-PCR法检测乳腺癌组织与正常组织中脂联素的表达差异,分析脂联素的表达与临床病理特征的关系。结果脂联素在乳腺正常组织中高表达(logFC>1,P<0.05);乳腺癌组织中脂联素阳性表达率为65.00%(52/80),低于癌旁组织中的阳性率91.25%(73/80),差异有统计学意义(P<0.05);乳腺癌组织中脂联素mRNA相对表达量为(0.683±0.317),高于癌旁组织中的(0.493±0.294),差异有统计学意义(P<0.05)。不同年龄、月经史、ER、PR、HER2、Ki-67表达间脂联素表达比较,差异无统计学意义(P>0.05);不同肿瘤直径、淋巴结转移、组织学分级、临床分期间脂联素表达比较,差异有统计学意义(P<0.05)。结论脂联素在乳腺组织中呈高表达,乳腺癌组织中呈低表达,与乳腺癌预后密切相关,有助于乳腺癌的诊断及预后评估。

Objective To study the expression of adiponectin in breast cancer tissues and its relationship with clinicopathological characteristics.Methods Based on the breast cancer data in the Cancer Genome Atlas(TCGA),combined with the angiogenesis gene set derived from AmiGO2,the factor adiponectin,which is related to breast cancer angiogenesis,was screened out.The GEPIA2 database was used to analyze the cancer genome atlas database,and combined with 80 cases of cancerous tissues and adjacent tissues that were diagnosed as breast cancer by pathological examination in our hospital from November 2019 to August 2020,The difference of adiponectin expression in breast cancer tissues and normal tissues was detected by immunohistochemical S-P method and qRT-PCR method,and the relationship between adiponectin expression and clinicopathological characteristics was analyzed.Results Adiponectin was highly expressed in normal breast tissues(logFC>1,P<0.05);The positive expression rate of adiponectin in breast cancer tissue was 65.00%(52/80),which was lower than the positive rate in adjacent tissues of 91.25%(73/80),the difference was statistically significant(P<0.05);The relative expression of adiponectin mRNA in breast cancer tissue was(0.683±0.317),which was higher than that in adjacent tissues(0.493±0.294),the difference was statistically significant(P<0.05).There was no statistically significant difference in the expression of adiponectin among different ages,menstrual history,ER,PR,HER2,and Ki-67 expressions(P>0.05);Different tumor diameters,lymph node metastasis,histological grade,and adiponectin expression during clinical period were compared,the difference was statistically significant(P<0.05).Conclusion Adiponectin is highly expressed in breast tissues and low in breast cancer tissues.It is closely related to the prognosis of breast cancer and is helpful for the diagnosis and prognosis evaluation of breast cancer.