miR-381在胰腺癌患者组织及外周血的表达及临床价值

The Expression and Clinical Value of miR-381 in Tissues and Peripheral Blood of Patients with Pancreatic Cancer

目的探究miR-381在胰腺癌组织及外周血中的表达。方法选取2017年7月~2018年8月邯郸市第一医院及河北医科大学第二医院确诊的50例胰腺癌患者的组织及外周血清,另外选取患者的癌旁正常组织及同期50例健康体检者血清样本作为对照组。采用RT-qPCR技术检测胰腺癌组织、血清和健康者血清中miR-381的表达水平,分析miR-381的表达水平与胰腺癌患者临床病理特征的关系,评价miR-381诊断胰腺癌的价值。结果胰腺癌组织及血清中miR-381的表达低于对照组,差异有统计学意义(P<0.05);不同肿瘤分期、淋巴结转移的胰腺癌患者miR-381的表达比较,差异有统计学意义(P<0.05);不同性别、年龄的胰腺癌患者miR-381的表达比较,差异无统计学意义(P>0.05);胰腺癌组织miR-381诊断胰腺癌的敏感性、特异性为84.51%及80.34%,胰腺癌血清miR-381诊断胰腺癌的敏感性、特异性为82.31%及81.26%。结论miR-381在胰腺癌组织及血清中呈低表达,其参与了胰腺癌的发生发展,可作为新的潜在肿瘤标志物。

Objective To explore the expression of miR-381 in pancreatic cancer tissues and peripheral blood.Methods The tissues and peripheral serum of 50 patients with pancreatic cancer diagnosed in Handan First Hospital and the Second Hospital of Hebei Medical University from July 2017 to August 2018 were selected.In addition,50 cases of normal tissues adjacent to cancer and 50 cases of healthy subjects were selected as the control group during the same period.RT-qPCR technology was used to detect the expression level of miR-381 in pancreatic cancer tissue,serum and serum of healthy individuals,analyze the relationship between the expression level of miR-381 and the clinicopathological characteristics of patients with pancreatic cancer,and evaluate the value of miR-381 in the diagnosis of pancreatic cancer.Results The expression of miR-381 in pancreatic cancer tissue and serum was lower than that in the control group,the difference was statistically significant(P<0.05);The expression of miR-381 in pancreatic cancer patients with different tumor stages and lymph node metastasis was compared,the difference was statistically significant(P<0.05);There was no statistically significant difference in the expression of miR-381 in pancreatic cancer patients of different genders and ages(P>0.05);The sensitivity and specificity of pancreatic cancer tissue miR-381 in diagnosing pancreatic cancer were 84.51%and 80.34%,and the sensitivity and specificity of pancreatic cancer serum miR-381 in diagnosing pancreatic cancer were 82.31%and 81.26%.Conclusion miR-381 is low expressed in pancreatic cancer tissues and serum.It is involved in the occurrence and development of pancreatic cancer and can be used as a new potential tumor marker.