大豆抗倒伏相关性状全基因组关联分析

Genome-wide association analysis of lodging-related traits in soybean

研究以150份大豆种质为试验材料开展2年3点试验,在收获期测定抗倒伏相关性状,并利用RTMGWAS方法作关联分析,共检测到99个显著关联SNP位点,其中48个效应显著位点,解释0.28%~3.38%表型变异;89个与环境互作显著位点,解释0.53%~7.04%表型变异;其中,21个位点主效表型变异解释率高于1%;与6个倒伏相关性状显著关联SNP位点中,与茎粗显著关联SNP位点最多,与抗倒指数显著关联SNP位点最少;获得22个有功能注释基因,根据基因表达量和基因功能注释,推测3个基因(Glyma.18G149700、Glyma.04G244100、Glyma.18G011400)可作为与大豆抗倒伏相关候选基因。

In this study,150 soybean germplasm were used as experimental materials for 2-year and 3-point test,lodging-related traits were measured at harvest time,and the association analysis was conducted by RTM-GWAS method.There were 99 significantly associated SNP sites detected,48 loci with significant effect accounted for 0.28%-3.38%of phenotypic variation,89 loci with significant interaction with environment explained 0.53%-7.04%of phenotypic variation,21 of them explained more than 1%of phenotypic variation.Among SNP loci significantly associated with six lodging related traits,the SNP loci associated with stem diameter were the most,and those associated with lodging index were the least.A total of 22 genes with functional annotation were obtained.According to gene expression and gene function annotation,three genes(Glyma.18G149700,Glyma.04G244100,and Glyma.18G011400)could be considrerd as candidate genes related to soybean lodging resistance.