基质血管组分(SVF)临床分离技术研究

The Research of Clinical Separation of SVF(Stromal Vascular Fraction)

脂肪组织易获取、组织相容性好且对供体影响小,可作为获得成体干细胞的重要来源。基质血管组分(SVF)是从脂肪中分离出来的包括脂源性干细胞(ADSC)和基质细胞的异质性细胞群。SVF促进组织的修复和再生已被大量的临床实验所证实,尤其是在美容整形和组织修复中的应用。早期,SVF通过酶消化法获得,随着近年来在临床中扩大应用,为确保患者安全和质量可控,开发出新型自动分离设备。同时,为符合一些国家监管要求,避免酶的使用,采用非酶消化法获取SVF。因此,该文主要针对基于酶消化法和非酶消化法已经发表临床分离方法和上市的相关设备作详细论述。

Adipose tissue is an important source of stem cells because it is easy to access and has a good histocompatibility and little influence on donors.SVF(stromal vascular fraction)is a heterogeneous group of cells isolated from adipose tissue,including ADSC(adipose derived stem cell)and stromal cells.According to a large number of clinical trials,SVF has been proved that it can promote tissue to repair and regenerate,especially in cosmetic surgery and tissue repair.In the early stage,SVF was obtained by enzyme digestion.With the extensive application of SVF in clinical practice during recent years,in order to ensure patient safety and quality control,some new automatic separation equipment was developed.Meanwhile,in order to meet the regulatory requirements of some countries and avoid the use of enzyme,non-enzymatic digestion method becomes a way of obtaining SVF.Therefore,current essay is primary to make a detailed discuss about reported clinical separation ways and related equipments based on the enzyme digestion and non-enzyme digestion.