IL-4基因甲基化在支气管哮喘患儿及健康儿童中的表达差异

Expression Difference of IL-4 Gene Methylation in Children with Bronchial Asthma and Healthy Children

目的研究IL-4基因甲基化在支气管哮喘患儿及健康儿童中表达的不同。方法方便选取该院2016年3月—2019年2月收治的支气管哮喘患儿54例作为研究组,另选取该院2016年3月—2019年2月进行门诊体检的健康儿童54名作为对照组,用甲基化特异性聚合酶链反应检测IL-4基因甲基化。结果研究组的IL-4基因完全甲基化率为31.48%(17/54),未甲基化率为18.52%(10/54),甲基化与未甲基化的共存率为50.00%(27/54),对照组的IL-4基因完全甲基化率为14.81%(8/54),未甲基化率为44.44%(24/54),甲基化与未甲基化的共存率为40.74%(22/54)。研究组的甲基化率与对照组的甲基化率差异有统计学意义(χ^(2)=4.216、4.615、3.977,P<0.05),研究组的IL-4基因甲基化率高于对照组,差异有统计学意义(P<0.05)。结论IL-4基因甲基化的表达在支气管哮喘患儿的发病有一定影响。

Objective To study the expression of IL-4 gene methylation in children with bronchial asthma and healthy children.Methods 54 children with bronchial asthma admitted to the hospital from March 2016 to February 2019 were convenient selected as the study group,and 54 healthy children who underwent outpatient physical examination in the hospital from March 2016 to February 2019 were selected as the control group.Methylation specific polymerase chain reaction detects the methylation of IL-4 gene.Results The IL-4 gene complete methylation rate of the study group was 31.48%(17/54),the unmethylation rate was 18.52%(10/54),and the coexistence rate of methylation and unmethylation was 50.00%(27/54),the complete methylation rate of IL-4 gene in the control group was 14.81%(8/54),and the unmethylation rate was 44.44%(24/54).The coexistence of methylation and unmethylation The rate is 40.74%(22/54).The methylation rate of the study group was significantly different from that of the control group(χ^(2)=4.216,4.615,3.997,P<0.05).The methylation rate of IL-4 gene in the study group was higher than that of the control group.The difference was statistically significant(P<0.05).Conclusion The expression of IL-4 gene methylation has a certain influence on the onset of bronchial asthma in children.