基于网络药理学麻黄治疗肺炎的作用机制研究

Mechanism of Mahuang(Ephedrae Herba)in Treatment of Pneumonia Based on Network Pharmacology

目的基于网络药理学分析麻黄治疗肺炎的作用机制。方法使用TCMSP数据库收集麻黄的活性成分及靶点,通过uniprot网站筛选基因名称。使用Genecards及OMIM数据库筛选肺炎相关基因并与麻黄靶基因取交集。使用string网站构建蛋白互网络(PPI网络),并使用Cytoscape软件构建"麻黄-靶蛋白-靶基因-肺炎"网络,最后使用RGUI软件中CludterProfiler包进行GO富集分析和KEGG代谢通路分析。结果筛选麻黄靶点及靶基因得到21个成分的234个靶基因名称,与肺炎相关基因取交集后得到101个公共靶基因。"麻黄-靶蛋白-靶基因-肺炎"网络中degree值超过10的基因有PTGS1、NCOA2,麻黄成分有quercetin、kaempferol、luteolin、naringenin、beta-sitosterol、Stigmasterol。PPI网络中degree值50以上的有白细胞介素-6(IL-6)、表皮生长因子受体(EGFR)、半胱氨酸天冬氨酸蛋白酶(CASP3)、血管内皮生长因子A(VEGFA)、有丝分裂原激活蛋白激酶(MAPK8)、Myc原癌基因蛋白(MYC)。GO富集和KEGG富集发现麻黄-肺炎体系中生物过程参与最多,且细胞凋亡通路、TNF信号通路、PI3K-Akt通路、P53信号通路是主要参与的信号通路。结论麻黄-肺炎体系存在着多分子、多靶点、多通路的作用机制。麻黄可能是通过调控IL-6、细胞凋亡通路、TNF信号通路、PI3K-Akt通路、P53信号通路等干预体内多种生物过程的方式治疗肺炎。

Objective To analyze the mechanism of Mahuang(Ephedrae Herba)in the treatment of pneumonia based on network pharmacology.Methods The active components and targets of Mahuang(Ephedrae Herba)were collected using the TCMSP database,and the gene names were screened by the uniprot website.The Genecards and OMIM databases were used to screen pneumonia-associated genes and interface with Mahuang(Ephedrae Herba)target genes.The protein network was constructed using the string website(PPI network),and the"Mahuang(Ephedrae Herba)-target protein-target gene-disease"network construction was performed using Cytoscape software.Finally,the CludterProfiler package in RGUI software was used for GO enrichment analysis and KEGG path analysis.Results A total of 234 target gene names of 21 components were screened for Mahuang(Ephedrae Herba)target and target genes,and 101 common target genes were obtained after crossing the pneumonia-related genes.The"Mahuang(Ephedrae Herba)-target protein-target gene-pneumonia"network was constructed.The genes with a value of more than 10 were found to be PTGS1 and NCOA2,and the Mahuang(Ephedrae Herba)components were quercetin,kaempferol,luteolin,naringenin,beta-sitosterol and Stigmasterol.In the PPI network,there were interleukin-6(IL-6),epidermal growth factor receptor(EGFR),caspase(CASP3),vascular endothelial growth factor A(VEGFA)and mitosis.Pro-activated protein kinase(MAPK8)and Myc proto-oncogene protein(MYC).GO enrichment and KEGG enrichment analysis revealed that the biological processes involved in the Mahuang(Ephedrae Herba)-pneumonia system were most involved,and the apoptotic pathway,TNF signaling pathway,PI3 K-Akt pathway and P53 signaling pathway were the main signaling pathways involved.Conclusion Mahuang(Ephedrae Herba)may treat pneumonia by regulating IL-6,apoptotic pathway,TNF signaling pathway,PI3 K-Akt pathway,P53 signaling pathway and other biological processes in vivo.And the Mahuang(Ephedrae Herba)-pneumonia system has a multi-molecule,multi-target,multi-channel mechanism of action.