厄他培南与米卡芬净合用对大鼠体内米卡芬净游离浓度的影响

Effects of Ertapenem Combined with Micafungin on the Free Concentration of Micafungin in Rats

目的:考察厄他培南对米卡芬净在大鼠体内游离浓度的影响。方法:SD大鼠40只,随机分为2组,每组20只,单独给药组大鼠尾静脉注射给予米卡芬净15 mg·kg^(-1),合并给药组大鼠尾静脉注射给予厄他培南100 mg·kg^(-1)和米卡芬净15 mg·kg^(-1)。采用离心超滤法结合超高效液相色谱法(UPLC)测定米卡芬净单独给药组与米卡芬净和厄他培南合并给药组大鼠血浆中游离米卡芬净的质量浓度,比较2组的药动学参数。结果:米卡芬净在20~640μg·L^(-1)范围内的线性关系良好(r=0.999 9)。米卡芬净绝对回收率为(90.61±3.29)%~(96.83±1.59)%(n=5),相对回收率为(94.05±4.12)%~(99.52±2.10)%(n=5)。厄他培南可使米卡芬净的Cmax升高且差异具有统计学意义(P<0.05),对其余参数无明显影响(P>0.05)。结论:本方法可以用于测定大鼠体内米卡芬净游离浓度。厄他培南对米卡芬净在大鼠血浆中的游离药物浓度有一定的影响。

Objective: To investigate the influence of ertapenem on the free concentration of micafungin in rats.Methods: Forty SD rats were randomly divided into two groups with 20 rats in each group.The rats in the single administration group were given micafungin 15 mg·kg^(-1) via caudal vein,while those in the combined administration group were given ertapenem 100 mg·kg^(-1) and micafungin 15 mg·kg^(-1).The concentration of free micafungin in plasma of rats was determined by centrifugal ultrafiltration combined with UPLC,and the pharmacokinetic parameters of the two groups were compared.Results: The linear range of micafungin was 20-640 μg·L^(-1)(r = 0.999 9).The absolute recovery of micafungin was(90.61±3.29) %-(96.83±1.59) %,and the relative recovery was(94.05±4.12) %-(99.52±2.10) %.Ertapenem could increase the Cmaxof micafungin(P< 0.05),while the other parameters had no statistically significant changes(P>0.05).Conclusion: The method can be used to determine the free concentration of micafungin in rats.Ertapenem has a certain effect on the free concentration of micafungin in rat’s plasma.