川芎对D-半乳糖衰老模型小鼠的抗衰老作用研究

Anti-senescence mechanism of Rhizoma Ligustici Chuanxiong in a mouse model of senescence induced by D-galactose

目的:研究川芎对D-半乳糖衰老模型小鼠抗衰老的作用机制。方法:将50只小鼠随机分为正常对照组10只和造模组40只,采用颈背部皮下注射D-半乳糖溶液的方法建立小鼠衰老模型,再将其分为衰老模型组及川芎低、中、高剂量组,每组各10只。药物干预50 d后检测小鼠脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、单胺氧化酶(MAO)。结果:与正常对照组比较,衰老模型组脑组织中SOD、GSH-Px含量降低,MDA、MAO含量升高,差异均有统计学意义(P<0.05);与衰老模型组比较,川芎低、中、高剂量组SOD、GSH-Px含量增加,MDA、MAO含量降低,差异均有统计学意义(P<0.05)。结论:川芎可增加脑组织中SOD、GSH-Px含量,降低MDA、MAO含量,具有良好的延缓衰老的作用。

Objective:To investigate the anti-senescence mechanism of Rhizoma Ligustici Chuanxiong in a mouse model of senescence induced by D-galactose.Methods:A total of 50 mice were randomly divided into normal control group with 10 mice and modeling group with 40 mice.A mouse model of senescence was established by subcutaneous injection of D-galactose solution at the neck and back,and then the modeling group was further divided into senescence model group and low-,middle-,and high-dose Rhizoma Ligustici Chuanxiong groups,with 10 mice in each group.The levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),and monoamine oxidase(MAO)in brain tissue were measured after 50 days of drug intervention.Results:Compared with the normal control group,the senescence model group had significant reductions in SOD and GSH-Px and significant increases in MDA and MAO in brain tissue(P<0.05).Compared with the senescence model group,the low-,middle-,and high-dose Rhizoma Ligustici Chuanxiong groups had significant increases in SOD and GSH-Px and significant reductions in MDA and MAO in brain tissue(P<0.05).Conclusion:Rhizoma Ligustici Chuanxiong can increase the levels of SOD and GSH-Px and reduce the levels of MDA and MAO in brain tissue and thus has a good effect in delaying senescence.