腐败血液中自生醇现象及法医学应用研究

Study on autogenous alcohol in putrefied blood and its forensic application

目的检测分析腐败血液中乙醇、甲醇等物质的生成过程,为正确判断案发时人血液中醇类物质的实际浓度提供实验依据。方法以正常健康人血液制作腐败样本,分别模拟人死亡后正常人血液和糖尿病人高糖血液的腐败过程,借助顶空气相色谱仪测定两种血液腐败后醇/醛类物质的生成情况并对比含量差异。结果相同实验条件下,高糖血液较正常健康血液更早腐败产生乙醇,但前者乙醇浓度低于后者;血液腐败过程中还会产生乙醛、甲醇、异丙醇、正丙醇等物质,其中正丙醇含量与乙醇生成量存在一定相关性。结论血液腐败能够产生乙醇、甲醇等多种物质。研究还为糖尿病人血液腐败产生醇类物质的差异性评价提供了依据。

Objective To detect and analyze the formation process of ethanol,methanol and other substances in putrefied blood,so as to provide experimental basis for correctly judging the actual concentration of alcohol substances in human blood at the time of crime.Method Putrefaction samples were made from normal human blood,and the putrefaction process of healthy blood of normal people and high glucose blood of diabetic patients after death were simulated respectively.The formation of alcohol/aldehydes in two kinds of blood after putrefaction was determined by headspace gas chromatography and the difference in content was compared.Results Under the same experimental conditions,high-glycemic blood produced ethanol earlier than normal healthy blood,but the ethanol concentration of the former was lower than that of the latter;acetaldehyde,methanol,isopropanol,n-propanol,etc.were also produced during blood putrefaction,and there was a certain correlation between the content of n-propanol and ethanol production.Conclusion Blood corruption can produce alcohol,methanol and other substances.The study also provide a basis for differential evaluation of alcoholic substances produced by blood stasis in diabetic patients.