长链非编码RNA胃癌高表达转录本1、胰岛素样生长因子2 mRNA结合蛋白1在结直肠癌中的表达及预后影响

Expressions of lncRNA GHET1 and IGF2BP1 in colorectal cancer and their influence on prognosis

目的观察长链非编码RNA胃癌高表达转录本1(lncRNA GHET1)、胰岛素样生长因子2 mRNA结合蛋白1(IGF2BP1)在结直肠癌(CRC)组织中的表达,探讨其与CRC患者预后的相关性。方法选取2014年1月至2015年1月于上海交通大学附属第六人民医院南院行手术治疗、具有完整病理及临床资料的CRC患者组织标本90例,设为研究组,另取该组手术患者癌旁正常组织标本作为对照组。实时荧光定量PCR法(qPCR)及免疫组织化学法检测两组标本中GHET1、IGF2BP1的表达情况;分析CRC组织GHET1、IGF2BP1表达情况与患者临床病理特征的关系;Kaplan-Meier法分析GHET1、IGF2BP1在CRC组织中的表达情况与预后情况的关系;Cox回归分析影响CRC预后情况的因素。结果研究组标本中的GHET1 mRNA水平及IGF2BP1蛋白阳性表达率均高于对照组(P<0.05);GHET1、IGF2BP1表达情况与肿瘤组织分化程度、浸润深度、TNM分期、淋巴结转移均有关(P<0.05);Kaplan-Meier分析结果显示,GHET1 mRNA高表达及IGF2BP1蛋白阳性患者生存率均低于GHET1 mRNA低表达及IGF2BP1蛋白阴性患者(32.80%vs 75.00%,Log-rankχ^(2)=17.775,P<0.05;33.33%vs 80.00%,Log-rankχ^(2)=13.615,P<0.05);多因素Cox分析显示,GHET1 mRNA高表达、IGF2BP1蛋白阳性表达是影响CRC预后情况的危险因素(P<0.05)。结论CRC患者癌组织中存在GHET1 mRNA水平及IGF2BP1蛋白阳性高表达,且与患者的不良预后密切相关。

Objective To investigate the expressions of long non-coding RNA gastric carcinoma highly expressed transcript 1 (lncRNA GHET1) and insulin-like growth factor Ⅱ mRNA-binding protein 1 (IGF2BP1) in colorectal cancer (CRC),and to explore their relationship with the prognosis of CRC.Methods From January 2014 to January 2015,tissue samples from 90 CRC patients with complete pathological and clinical data in South Hospital of the Sixth People’s Hospital Affiliated to Shanghai Jiaotong University were selected as the study group,and the adjacent tissue samples were taken as the control group.The expressions of GHET1 and IGF2BP1 were detected by real-time fluorescence quantitative PCR (qPCR) and immunohistochemistry.The relationship between the expressions of GHET1 and IGF2BP1 and clinicopathological features was analyzed.Kaplan-Meier method was used to analyze the relationship between the expressions of GHET1 and IGF2BP1 in CRC tissues and prognosis,while Cox regression analysis was used for the prognostic factors of CRC.Results The level of GHET1 mRNA and the positive expression rate of IGF2BP1 protein in the study group were significantly higher than those in the control group (P<0.05).The expressions of GHET1 mRNA and IGF2BP1 protein were correlated with tumor differentiation,depth of invasion,TNM stage and lymph node metastasis (P<0.05).Kaplan-Meier analysis showed that the survival rate of patients with high expression of GHET1 mRNA and IGF2BP1 protein positive was lower than patients with low expression of GHET1 and IGF2BP1 negative (32.80% vs 75.00%,χ2=17.775;33.33% vs 80.00%,χ2=13.615;both P<0.05).Multivariate Cox analysis showed that high expression of GHET1 mRNA and positive expression of IGF2BP1 protein were risk factors for the prognosis of CRC (P<0.05).Conclusion There are high expressions of GHET1 mRNA and IGF2BP1 protein in CRC tissues,which are closely related to the poor prognosis of patients.