碱性成纤维细胞生长因子凝胶缓解氯倍他索反复涂抹联合胶带剥离术导致的小鼠皮损

bFGF gel ameliorates mouse skin injury induced by successive application of clobetasol combined with tape stripping

目的探究碱性成纤维细胞生长因子对氯倍他索诱导的小鼠皮损的作用。方法将C57BL/6J小鼠背部反复涂抹氯倍他索,30 min后给予bFGF凝胶,然后行胶带剥离术,记录小鼠的背部皮肤外观、检测表皮失水率以及对皮肤组织进行病理分析。通过MTT法和免疫印迹法检测氯倍他索刺激的人角质形成细胞系HaCaT的细胞活力及紧密连接蛋白表达。结果与溶媒对照组相比,造模小鼠皮肤明显损伤,表皮失水率增高,H&E染色显示表皮细胞大量死亡,伴随真皮层炎性浸润和局部出血。涂抹bFGF凝胶后小鼠皮损、表皮失水率显著低于未干预小鼠,同时H&E染色显示表皮细胞死亡、真皮层炎性浸润和血管数目减少。此外,氯倍他索刺激抑制HaCaT增殖和紧密连接蛋白Claudin 1,Claudin 2的表达。结论给予bFGF凝胶显著减弱氯倍他索反复涂抹联合胶带剥离术导致的小鼠皮肤损伤,可能为临床上激素依赖引起的皮肤损伤提供潜在的治疗方案。

Objective To investigate the role of basic fibroblast growth factor in mouse skin injury induced by clobetasol.Methods Skin damage was induced by continuously topical administration with clobetasol followed by tape stripping in C57BL/6J mice′s dorsal skin.bFGF containing gel was applied at 30 min after clobetasol treatment.Dorsal skin image and trans-epidermal water loss(TEWL)were recorded,H&E staining was conducted in skin tissue with further histological analysis.Cell viability and tight junction protein expressions in human keratinocyte cell line,HaCaT were also determined via MTT assay and western blotting respectively.Results Compared with negative control group,skin injury was confirmed in clobetasol treated mice by rash,edema and increased TEWL,accompanied by shedding and death of epidermic cells and infiltration around the dermal layer in H&E stained skin tissue slides.However,bFGF containing gel treatment significantly attenuated skin damage and TEWL.We also noticed reduced death of epidermic cells,infiltration around the dermal layer and hemorrhage.Moreover,clobetasol stimulation mediated inhibition of cell proliferation and downregulation of tight junction protein,Claudin 1 and Claudin 2 in HaCaT in vitro.Conclusion bFGF containing gel could effectively alleviate mouse skin injury induced by successive application of clobetasol combined with tape stripping,thus provides prospective therapeutic approach for clinic corticosteroid addiction associated side effects.