摘要
2018年在北京通州试验基地的一温室内发生一种甜瓜新症状病害,为了能够有效地控制该病害,采集典型发病植株进行病原菌分离纯化,测定其致病性;利用形态学特征和rDNA ITS序列分析法鉴定该病原菌种类,并测定其部分生物学特性。结果表明,该病原菌为尖孢镰刀菌甜瓜专化型Fusarium oxysporum f.sp melonis。该病原菌在查彼克培养基(Czapek)上生长最快,最适生长温度为25~30℃,比较适合的碳源为甘露醇、麦芽糖和葡萄糖,比较适合的氮源为酵母浸膏。该病原菌对光照不敏感,菌丝致死温度为68℃下10 min。通过分析病害的发生特点,推测这种病害初侵染源可能是种子携带的病原菌。
A new symptom disease of melon(Cucumis melo L.)was observed in a greenhouses in Tongzhou,Beijing in 2018.In order to promote the study of controlling measures to the disease,the typical diseased plants were collected and the pathogen was isolated,and the pathogen was identified on its morphology and rDNA ITS sequence analysis.The biological characteristics of the pathogen growth were also studied.The results showed that the pathogen of this disease was Fusarium oxysporum f.sp melonis.The pathogen grows fastest on Czapek medium.The optimal temperature for the pathogen to grow on PDA is 25-30℃.The pathogen grows faster with mannitol,maltose,and glucose as carbon sources,and with yeast extract as nitrogen sources.The pathogen was insensitive to light.The mycelium lethal temperature is 68℃for 10 minutes.By analyzing the characteristics of this disease,the primary infection source of the disease may come from the seeds with the pathogen.
作者
耿丽华
王建设
马建
杨洋
史越
宋顺华
GENG Lihua;WANG Jianshe;MAJian;YANG Yang;SHI Yue;SONG Shunhua(Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of North China Area,Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Vegetable Germplasm Improvement,Ministry of Agriculture and Rural Affairs/Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China)
出处
《中国瓜菜》
CAS
北大核心
2021年第3期15-20,共6页
China Cucurbits And Vegetables
基金
国家重点研发计划(2018YFD0201300)
国家现代农业产业技术体系北京市西甜瓜创新团队专项(BAIC10)
北京市农林科学院科技创新能力建设专项(KJCX20200113)。
关键词
甜瓜
病害新症状
病原鉴定
尖孢镰刀菌
生物学特性
Melon
New symptom disease
Pathogen identification
Fusarium oxysporum
Biological characteristics
