三种检测方法检测抗dsDNA抗体的临床意义

The clinical significance of three detection methods to detect anti-dsDNA antibodies

目的探究将绿蝇短膜虫间接免疫荧光法、欧蒙酶免疫斑点法以及胶体金快速斑点法应用于检测抗双链DNA(dsDNA)抗体中的效果。方法120例系统性红斑狼疮患者,根据病情不同分为疾病活动组及疾病稳定组,每组60例;同时选取60例健康体检者作为对照组。分别采用绿蝇短膜虫间接免疫荧光法、欧蒙酶免疫斑点法以及胶体金快速斑点法对三组研究对象的抗双链DNA抗体进行检测,分析三种检测方式的检测阳性率。结果绿蝇短膜虫间接免疫荧光法对疾病活动组患者抗双链DNA抗体的检测阳性率为95.00%(57/60),对疾病稳定组患者的检测阳性率为71.67%(43/60),对对照组的检测阳性率为5.00%(3/60);欧蒙酶免疫斑点法对疾病活动组患者抗双链DNA抗体的检测阳性率为76.67%(46/60),对疾病稳定组患者的检测阳性率为61.67%(37/60),对对照组的检测阳性率为10.00%(6/60);胶体金快速斑点法对疾病活动组患者抗双链DNA抗体的检测阳性率为80.00%(48/60),对疾病稳定组患者的检测阳性率为65.00%(39/60),对对照组的检测阳性率为8.33%(5/60)。三种检测方法对疾病活动组患者抗双链DNA抗体的检测阳性率高于疾病稳定组、对照组,疾病稳定组高于对照组,差异具有统计学意义(P<0.05);绿蝇短膜虫间接免疫荧光法对系统性红斑狼疮患者抗双链DNA抗体的检测阳性率为83.33%(100/120),均高于欧蒙酶免疫斑点法的69.17%(83/120)、胶体金快速斑点法的72.50%(87/120),差异具有统计学意义(P<0.05)。结论三种诊断方式对于抗双链DNA抗体均具有较高的诊断阳性率,并且在对患者的各个时期进行诊断时,产生的诊断效果较为良好,而三种诊断方式应用于患者的诊断中,可以选择联合诊断,这样有助于避免误诊或者漏诊。

Objective To investigate the effect of crithidia luciliae immunofluorescence test,dot imrnunogold filtration assay,Euroimmun dot immunobinding assay in the detection of anti-double-stranded DNA(dsDNA)antibodies.Methods A total of 120 patients with systemic lupus erythematosus were divided into disease-active group and disease-stable group according to their different conditions,with 60 cases in each group.At the same time,60 healthy subjects were selected as the control group.The anti-double-stranded DNA antibodies of the three groups were detected by crithidia luciliae immunofluorescence test,dot imrnunogold filtration assay,Euroimmun dot immunobinding assay respectively,and the diagnostic positive rates of the three detection methods were analyzed.Results According to ccrithidia luciliae immunofluorescence test,the positive rate of anti-double-stranded DNA antibodies in the disease-active group was 95.00%(57/60),which was 71.67%(43/60)in disease-stable group and 5.00%(3/60)in the control group;according to Euroimmun dot immunobinding assay,the positive rate of anti-double-stranded DNA antibodies in the disease-active group was 76.67%(46/60),which was 61.67%(37/60)in disease-stable group and 10.00%(6/60)in the control group;according to dot imrnunogold filtration assay,the positive rate of anti-double-stranded DNA antibodies in the disease-active group was 80.00%(48/60),which was 65.00%(39/60)in disease-stable group and 8.33%(5/60)in the control group.The positive rate of anti-double-stranded DNA antibodies of the three detection methods in the disease-active group was higher than that of the disease-stable group and the control group,and the diseasestable group was higher than the control group,and the difference was statistically significant(P<0.05).In patients with systemic lupus erythematosus,the positive rate of anti-double-stranded DNA antibodies was 83.33%(100/120)by crithidia luciliae immunofluorescence test,which was higher than 69.17%(83/120)by Euroimmun dot immunobinding assay,and 72.50%(87/120)by dot imrnunogold filtration assay,and the difference was statistically significant(P<0.05).Conclusion The three diagnostic methods all have a high diagnostic positive rate for anti-double-stranded DNA antibodies,and the diagnosis accuracy is good in each period of the patient,and combined diagnosis of three methods can be selected to help avoid misdiagnosis or missed diagnosis.