临床病原微生物检验标本不合格的相关因素及控制措施分析

Analysis of related factors and control measures for the unqualified specimens of clinical pathogenic microorganisms

目的分析临床病原微生物检验标本不合格的原因及质控策略。方法选取1000份病原微生物标本作为调查对象,以回顾性分析的方法统计所有病原微生物标本的检验结果,分析病原微生物检验不合格标本的发生情况及不合格的原因,总结控制措施。结果不合格标本84份,占比为8.40%;其中体液标本50份,占比为5.00%;血标本30份,占比为3.00%;一次性标本4份,占比为0.40%。标本不标准、标本污染、储存标本方式错误、储存标本时间超长、标本处理方式错误、检验人员技术不达标是导致病原微生物标本检验不合格的单因素(P<0.05)。Logistic回归分析显示,标本不标准、标本污染、标本储存错误、标本储存时间超长、标本处理方式错误、检验人员技术不达标为临床病原微生物检验不合格的独立危险因素(P<0.05)。结论临床病原微生物标本检验不合格的原因表现为多个方面,因此需要从多角度总结相关因素,进行恰当的标本处理,才能从根本上提升标本检验的准确性和合格率,为患者及时治疗提供依据。

Objective To analyze the reasons and quality control strategies for the unqualified specimens of clinical pathogenic microorganisms.Methods 1000 samples of pathogenic microorganisms were selected as the investigation objects.The results of all the samples were analyzed retrospectively.The occurrence and causes of unqualified samples were analyzed,and the control measures were summarized.Results There were 84 unqualified samples,accounting for 8.40%,including 50 body fluid samples,accounting for 5.00%,30 blood samples,accounting for 3.00%,and 4 disposable samples,accounting for 0.40%.Non-standard specimens,specimen contamination,wrong specimen storage methods,excessively long specimen storage time,wrong specimen handling methods,and technical failure of inspectors are the single factors that lead to unqualified microorganism specimen(P<0.05).Logistic regression analysis showed that non-standard specimens,specimen contamination,specimen storage errors,excessively long specimen storage time,wrong specimen handling methods,and technical failure of inspectors were independent risk factors for clinical pathogenic microorganism specimen inspection failure(P<0.05).Conclusion There are many reasons for the unqualified clinical pathogenic microorganism specimens.Therefore,it is necessary to summarize the relevant factors from multiple angles and conduct appropriate specimen processing in order to fundamentally improve the accuracy and pass rate of specimen inspections and provide a basis for timely treatment of patients.