木尼孜其赛甫拉合剂质量标准研究

Study on quality standard of Muniziqisaifula Mixture

目的建立木尼孜其赛甫拉合剂的质量标准。方法采用薄层色谱法(TLC)对睡莲花、菊苣进行定性鉴别;采用高效液相色谱法测定秦皮乙素的含量,色谱柱为Inertsil ODS-SP(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-0.2%H3P04(9∶6∶85),流速为1.0 mL/min,柱温为30℃,进样量为10μL,检测波长为342 nm。结果TLC法能准确鉴别睡莲花、菊苣,分离效果好,斑点清晰;秦皮乙素在5.09~163.04μg/mL(r=1.0000)范围内线性关系良好,平均回收率为100.99%。结论本方法专属性强、重复性好,可用于木尼孜其赛甫拉合剂的质量控制。

Objective To establish quality standards for Muniziqisaifula Mixture.Methods Thin layer chromatography(TLC)was used to identify Nymphaea candida Presl.and Cichorium intybus L.The conten of esculetin in mixture was determined by high performance liquid chromatography.The Inertsil ODS-SP(250 mm×4.6 mm,5μm)was used as the colum,the mobile phase was quercetin took methanol-acetonitrile-0.2%H3PO4(9∶6∶85),the flow rate was 1.0 mL/min,the sample size was 10μL,the column temperature was 30℃and the detection wavelength was set at 342 nm.Results Nymphaea candida Presl.and Cichorium intybus L.could be clearly detected by TLC.The esculetin had a good linear relationship between 5.09-163.04μg/mL(r=1.0000).The average recovery rates of esculetin was 100.99%.Conclusion This method is fast and can be used to control the quality of Muniziqisaifula Mixture.