基于网络药理学探讨柴胡疏肝散治疗慢性前列腺炎的作用机制

Exploration on the mechanism of Chaihu Shugan Powder in the treatment of chronic prostatitis based on network pharmacology

目的基于网络药理学探究柴胡疏肝散治疗慢性前列腺炎的作用机制。方法根据类药性及口服生物利用度,通过中药系统药理数据库和分析平台、GeneCards筛选柴胡疏肝散治疗慢性前列腺炎的有效活性成分预测作用靶标;采用Cytoscape 3.7.2绘制出柴胡疏肝散活性成分-慢性前列腺炎作用靶标网络图形;应用STRING数据库制作靶标相互作用网络分析;最终在生物学信息注释数据库中进行靶点、信号通路、生物过程分析。结果共筛选出129个主要活性成分,其中陈皮5个、柴胡14个、川芎6个、香附17个、枳壳5个、白芍11个、甘草71个。得到活性成分的作用靶标1121个,包括IL-6、IL-2、SRC、EGFR、IGF1R等;KEGG通路富集分析结果显示,主要涉及趋化因子信号通路、MAPK信号通路、T细胞信号通路等。结论本研究揭示了柴胡疏肝散治疗慢性前列腺炎的潜在靶点、KEGG信号通路,可为其干预慢性前列腺炎的作用机制提供新思路。

Objective To explore the mechanism of Chaihu Shugan Powder in the treatment of chronic prostatitis based on network pharmacology.Methods According to the drug-likeness and oral bioavailability,the predictive targets of effective active ingredients of Chaihu Shugan Powder in the treatment of chronic prostatitis were screened through the pharmacology database analysis platform of traditional Chinese medicine system and GeneCards.The network graph of active component of Chaihu Shugan Powder-target of chronic prostatitis was drawn by Cytoscape 3.7.2.The network analysis of target interaction was made by STRING database.Finally,the target,signal pathway and biological process were analyzed in the biological information annotation database.Results A total of 129 active components were screened out,among which,the number of Citri Reticulatae Pericarpium,Bupleuri Radix,Chuanxiong Rhizoma,Cyperi Rhizoma,Aurantii Fructus,Paeoniae Radix Alba,Glycyrrhizae Radix Et Rhizoma was 5,14,6,17,5,11,71 respectively.A total of 1121 targets of active ingredients were obtained,including IL-6,IL-2,SRC,EGFR,IGF1R,and so on.KEGG pathway enrichment analysis showed that chemokine signaling pathway,MAPK signaling pathway,T cell signaling pathway and others were mainly involved.Conclusion This study reveals the potential target and KEGG signaling pathway of Chaihu Shugan Powder in the treatment of chronic prostatitis,which can provide new ideas for the mechanism of its intervention in chronic prostatitis.