柳树种质核DNA含量的流式测定

Evaluation of nuclear DNA content in willow germplasm by flow cytometry

核DNA含量(C值)是物种生物学特性的重要特征之一。为探明柳树不同种质间核DNA含量及变化规律,该研究以柳树14个自然种和8个品种为材料,利用水稻和大豆为内标,基于流式细胞仪检测并分析了每份种质的核DNA含量(1C值)。结果表明,22份柳树种质核DNA含量1C值变化范围为0.38—0.90 pg。14个自然种中,龙爪柳核DNA含量1C值最大,为0.67 pg,蒿柳核DNA含量1C值最小,为0.38 pg。8个品种中,苏柳932核DNA含量1C值最大,为0.90 pg,苏柳1053核DNA含量最小,为0.39 pg。多重比较及方差分析表明:柳树大部分种质个体间核DNA含量1C值存在显著差异(P<0.05),且不同类型乔木组和灌木组间存在极显著差异(P<0.01)。该研究结果不但可丰富柳属植物C值数据库,还可为柳属系统发育及基因组学研究奠定基础。

The nuclear DNA amount(C value)is a significant feature of biological characters for species.In order to evaluating the DNA contents and variation patterns among different willow germplasm,each 1C value for 14 nature species and 8 varieties was detected and analyzed based on flow cytometry(FCM)using rice and soybean as internal reference.The results demonstrated that the nuclear DNA contents of 22 germplasm ranged from 0.38 to 0.90 pg.Among 14 nature species,the largest 1C value with 0.67 pg was found in Salix matsudana f.tortusoa and the smallest 1C value with 0.38 pg was screened in S.viminalis.Among 8 varieties,S.×jiangsuensis‘J932’had the largest 1C value with 0.90 pg and S.×jiangsuensis‘J1053’had the smallest 1C value with 0.39 pg.Furthermore,there were significant differences among individuals of most willow germplasm(P<0.05)and extremely significant differences between tree willow and shrub willow groups(P<0.01).This study can provide novel resources for C value database of willow and can lay a foundation for future research of phylogeny and genomics in willow.