三氯化铁絮凝小球藻的采收工艺

Harvesting Technology of Chlorella sorokiniana Using FeCl_(3)as a Flocculant

【目的】研究三氯化铁对小球藻絮凝采收效果的影响,并优化采收工艺条件。【方法】分析FeCl_(3)浓度、小球藻初始浓度和絮凝时间对小球藻采收效率影响,利用响应面法优化利用FeCl_(3)采收小球藻的工艺参数。【结果】单因素实验表明,FeCl_(3)质量浓度为0.6 g/L、小球藻初始质量浓度为0.42 g/L和絮凝时间为30 min时,小球藻的采收效率最高。响应面法分析表明,小球藻初始浓度对小球藻采收率有显著影响(P<0.05);影响采收效率的主次因素顺序为小球藻初始浓度>FeCl_(3)浓度>絮凝时间;FeCl_(3)浓度和小球藻初始浓度的交互作用对小球藻的采收效率有显著影响(P<0.05);FeCl_(3)浓度二次项对小球藻的采收效率有极显著影响(P<0.01)。【结论】利用FeCl_(3)采收小球藻的最佳工艺条件:小球藻初始质量浓度为0.55 g/L,FeCl_(3)质量浓度为0.6 g/L,絮凝时间为38 min,此时的采收效率为(98.6±1.9)%。

【Objective】The effects of FeCl_(3)on flocculation and harvest technology of Chlorella sorokiniana were studied,and the harvesting process conditions were optimized in this work.【Method】Effects of FeCl_(3)concentrations,initial concentrations of C.sorokiniana,and flocculation time on the harvesting efficiency of C.sorokiniana were firstly analyzed,and then the technological parameters for harvesting this alga using FeCl_(3)were optimized by the response surface methodology.【Result】Single factor experiments showed that the maximum harvesting efficiency of C.sorokiniana was obtained when the FeCl_(3)concentration was 0.6 g/L,and initial concentration of C.sorokiniana was 0.42 g/L,and flocculation time was 30 min.Response surface methodology analysis suggested that initial concentrations of C.sorokiniana had a significant effect on the harvesting efficiency of this alga(P<0.05);the order of factors affecting the harvesting efficiency was the initial concentrations of C.sorokiniana>FeCl_(3)concentrations>flocculation time;interaction effects of FeCl_(3)concentrations and initial concentrations of C.sorokiniana on the harvesting efficiency of this alga were significant(P<0.05);and quadratic effects of FeCl_(3)concentrations on the harvesting efficiency of this alga were extremely remarkable(P<0.01).【Conclusion】The optimum technological conditions for harvesting C.sorokiniana using FeCl_(3)were determined as follows:initial concentration of C.sorokiniana was 0.55 g/L,and FeCl_(3)concentration was 0.6 g/L,and flocculation time was 38 min,and the maximum harvesting efficiency(98.57±1.85)%was obtained under the above optimum technological conditions.