响应面法优化艳山姜多糖提取工艺及抗氧化研究

Optimization of Extraction Process and Antioxidant Activity of Polysaccharides from Alpinia zerumbet (Pers.) Burtt. et Smith by Response Surface Methodology

以艳山姜为试验原料,采用响应面法优化艳山姜多糖提取工艺,并研究艳山姜多糖的抗氧化活性。建立以葡萄糖为对照品,紫外分光光度法测定多糖含量的定量分析方法。在单因素试验基础上,以提取时间、超声功率和液料比为自变量,多糖得率为因变量,运用Box-Behnken设计-响应面优化艳山姜多糖的提取工艺。通过对DPPH自由基、超氧阴离子自由基、羟基自由基清除作用研究艳山姜多糖的抗氧化活性。结果表明,艳山姜多糖最佳提取工艺条件为:提取时间35 min、超声功率70 W、液料比40∶1(mL/g),在此条件下多糖得率为5.37%。艳山姜多糖对DPPH自由基、超氧阴离子自由基和羟基自由基有较强的清除能力,多糖浓度越高,抗氧化活性越强。

Taking Alpinia zerumbet(Pers.)Burtt.et Smith as the test material,the response surface methodology was used to optimize the extraction process of Alpinia zerumbet polysaccharides,and the antioxidant activity of Alpinia zerumbet polysaccharides was studied.Ultraviolet spectrophotometry with glucose was used to establish a quantitative analysis method for the determination of polysaccharide content as the reference substance.On the basis of single factor experiment,taking extraction time,ultrasonic power and liquid-material ratio as independent variables,and polysaccharide yield rate as dependent variable,Box-Behnken design-response surface was used to optimize the extraction process of Alpinia zerumbet polysaccharides.The anti-oxidant activity of polysaccharide was studied by scavenging DPPH,superoxide anion and hydroxyl free radical.The results showed that the optimal extraction process conditions of Alpinia zerumbet polysaccharides were:extraction time 35 min,ultrasonic power 70 W,liquid-material ratio 40∶1(mL/g),and the polysaccharide yield was 5.37%.Polysaccharide had strong scavenging ability to DPPH,superoxide anion and hydroxyl free radicals.The higher the polysaccharide concentration,the stronger the antioxidant activity.