组织CXCL13和Dyrk1b表达预测卵巢癌预后的临床意义

Expressions of tissue CXCL13 and Dyrk1b and their clinical significance in predicting the prognosis of patients with ovarian cancer

目的观察组织CXCL13和双特异性酪氨酸磷酸化调节激酶1b(Dyrk1b)表达量在预测卵巢癌患者预后的临床价值。方法选择2012年1月至2016年1月行手术治疗的卵巢癌患者116例为卵巢癌组;选择同期行卵巢良性肿瘤98例和无病变区域的卵巢组织30例,分别为良性肿瘤组和对照组。3组的组织标本采用免疫组织化学法检测,比较3组表达CXCL13和Dyrk1b量的差别,卵巢癌组织表达CXCL13和Dyrk1b量与临床病理指标的关系,随访2年后生存组和死亡组的差异,预测死亡方面的灵敏度和特异性。结果卵巢癌组组织表达CXCL13和Dyrk1b明显高于良性卵巢瘤组和对照组(P<0.01),而良性肿瘤组明显高于正常对照组(P<0.01)。卵巢癌组织表达CXCL13和Dyrk1b量与分化程度、FIGO分期、淋巴结转移、腹腔积液、CA125水平和对化疗药物敏感性具有相关性(P<0.01),而与年龄、绝经、组织类型和肿瘤直径无相关性(P>0.05)。死亡组的卵巢癌组织CXCL13和Dyrk1b表达量明显高于生存组(P<0.01)。通过受试者曲线分析,发现CXCL13和Dyrk1b表达量在预测死亡具有较高的灵敏度和特异性,联合检测的灵敏度87.1%,特异性88.9%,曲线下面积明显优于单纯指标CXCL13(Z=2.200,P=0.028)和Dyrk1b(Z=2.573,P=0.010),而在预测死亡方面CXCL13和Dyrk1b差异无统计学意义(P>0.05)。结论CXCL13和Dyrk1b参与了卵巢癌的发生发展,联合检测组织CXCL13和Dyrk1b表达量对预后判断具有重要的临床价值。

Objective To investigate the expressions of tissue CXCL13 and bispecific tyrosine phosphorylation-regulated kinase 1b(Dyrk1b)and their clinical significance in predicting the prognosis of patients with ovarian cancer.Methods A total of 116 patients with ovarian cancer who were treated by surgery in our hospital from January 2012 to January 2016 were enrolled ovarian cancer group,at the same tim,the other 98 patients with ovarian benign tumor were enrolled as ovarian benign tumor group,and the 30 cases of normal ovary tissues were enrolled as control group.The expression levels of CXCL13 and Dyrk1b were detected by immunohistochemistry.Moreover the correlation between the expressions of CXCL13,Dyrk1b in ovarian cancer tissues and clinicopathological indexes was analyzed.After 2-year follow up,the differences between survival cae group and death case group were compared.Furthermore the sensitivity and specificity of detectoion of CXCL13 and Dyrk1b in predicting death of patients were observed.Results The expression levels of CXCL13 and Dyrk1b in ovarian cancer group were significantly higher than those in benign ovarian tumor group and control group(P<0.01),moreover,which in benign tumor group were significantly higher than those in control group(P<0.01).The expression levels of CXCL13 and Dyrk1b in ovarian cancer tissues were closely correlated with the differentiation degree,FIGO stage,lymph node metastasis,ascites,CA125 level and sensitivity to chemotherapy drugs(P<0.01),however,which were not correlated with patient’s age,menopause,tissue type(P>0.05).The expression levels of CXCL13 and Dyrk1b in ovarian cancer tissues in death case group were significantly higher than those in survival case group(P<0.01).In addition the detection of expression levels of CXCL13 and Dyrk1b had higher sensitivity and specificity in predicting death rate.The sensitivity of combined detection was 87.1%,specificity was 88.9%,and the area under curve was significantly better than that of CXCL13 and Dyrk1b(P<0.05),but there was no significant difference in predicting death rate between CXCL13 and Dyrk1b(P>0.05).Conclusion CXCL13 and Dyrk1b are involved in the pathogenesis and development of ovarian cancer,and the combined detection of CXCL13 and Dyrk1b has important clinical significance in prognosis evaluation.