富硒产品中硒的形态分析及化学评分模式的建立

Speciation analysis of selenium in selenium⁃enriched products and establishment of chemical scoring model

采用微波水解、HPLC⁃HG⁃AFS法测定了硒蛋白粉、硒蛋白片、肽粉、富硒原料等19种硒产品中的总硒、硒代氨基酸和亚硒酸根离子[Se(IV)],分析了硒代氨基酸、Se(IV)和其他形态硒占总硒的百分比及不同形态硒代氨基酸的组成比例。以此为依据,将硒产品分为硒蛋白型、单一硒代氨基酸型、其它形态硒型及有机无机硒混合型。根据DBS42/002⁃2014规定建立了富有机硒产品评分模式,其中18种为富有机硒产品;根据适硒地区母乳中硒代氨基酸的组成比例提出了硒代氨基酸的化学评分模式,评分结果显示13种以蛋白态硒为主的硒产品中硒代氨基酸的组成比例均与母乳相差甚远,不利于人体平衡吸收利用,其中10种SeMet含量远远超过人体所需,SeCys2为限制硒代氨基酸。该评分模式的建立对硒产品的开发具有指导意义。

Se⁃substituted amino acids,selenite ions[Se(IV)]and other forms of selenium in 19 selenium products including selenium protein powder,selenoprotein tablets,peptide powder and selenium⁃rich raw materials were deter⁃mined by microwave hydrolysis⁃HPLC⁃HG⁃AFS method.As a percentage of total selenium,selenium in protein,Se(IV)and other forms and the percentage of selenium amino acids in different structures were analyzed.On this basis,se⁃lenium products can be divided into selenoproteins,selenopamino acid,selenium forms and mixed selenium in organic and inorganic forms.Chemical scoring model was established on the basis of the regulation of DBS42/002⁃2014,the re⁃sult showed that 18 of 19 selenium products are rich organic selenium products.According to the proportion of seleno⁃ami⁃no acids in breast milk in suitable selenium area,the chemical grading standard of seleno⁃amino acids was proposed.Based on this standard,among the 13 selenoprotein⁃based selenium products,the selenoamino acid,SeCys2,is the limit⁃ing amino acid,and the SeMet was far more than that human need.In composition ratio,all seleno⁃amino acids are quite different from that of human milk,and are not conducive to the balanced absorption and utilization of human body.The establishment of the scoring model has a guiding effect in the development and evaluation of selenium products.