不同基质对茅苍术移栽组培苗叶片导水度及光合的影响

Effects of Different Substrates on Leaf Water Conductivity and Photosynthesis of Tissue Culture Plantlets of Atractylodes lancea

选取茅苍术(Atractylodes lancea(Thunb.)DC)组培苗为实验材料,进行驯化栽培,研究不同基质的物理性质,比较茅苍术组培苗在各基质中的叶片固有导水度、光合作用及生长状况,以筛选出最优炼苗基质。结果表明,基质持水特性是影响组培苗驯化的关键因素,T 3(蛭石∶珍珠岩∶泥炭土=1∶1∶2)基质中培养的驯化苗的叶片水分状况、光合及生长方面与其他处理相比表现较好,可作为茅苍术组培苗驯化的理想基质。基于生理电阻的叶片固有导水度可用于水分状况的及时诊断。研究结果为快速获得大量优势茅苍术种苗提供指导,同时为组培苗驯化炼苗提供理论基础和技术支撑。

In this study,the tissue culture seedlings of Atractylodes lancea(Thunb.)DC were selected as experimental materials for domestication and cultivation.The physical properties of different substrates were studied,and the inherent water conductivity,photosynthesis and growth status of the leaves of tissue culture seedlings of A.lancea were also compared in each substrate,so as to select the optimal substrate.The results showed that the water-holding capacity of substrate was the key factor affecting domestication of tissue culture seedlings.Compared with other treatments,T 3(vermiculite∶perlite∶peat=1∶1∶2)showed better leaf water,photosynthesis and growing status,and could be used as an ideal substrate for domestication of tissue culture seedlings of A.lancea.Leaf intrinsic water conductivity based on physiological resistance could be used for timely diagnosis of water status.The results of this study provided guidance for rapid acquisition of a large number of dominant A.lancea seedlings,and provided theoretical basis and technical support for domestication of tissue culture seedlings.