摘要
Despite the promising prospect of small interfering RNA(siRNA) for the treatment of diverse diseases,it remains challenging to develop novel delive ry materials to desired tissues and cells.In this study,a novel iron oxyhydroxide(FeOOH) nanoparticle(NP) whose surface was modified with branched polyetherimide(PEI) was developed to deliver siRNA into the cancer cells.It was demonstrated that PEI-FeOOH(PFeOOH) efficiently complexed siRNA,mediated effective cellular uptake and endosomal escape,thereby triggering robust gene silencing in vitro.In addition,PFeOOH/siRNA formulation loading with anti-RRM2 siRNA effectively inhibited the growth of tumor tissues,and exhibited excellent safety profiles in vivo.Therefore,this study conceptually provided a FeOOH-based nucleic acid delivery vesicle which can potentially use to achieve diagnosis and therapy simultaneously.
基金
supported by the Hunan Provincial Natural Science Foundation of China(Nos.2018JJ1019,2019JJ50196)
the Hu-Xiang Young Talent Program(No.2018RS3094)
the National Natural Science Foundation of China(Nos.31871003,31901053)
the Beijing Institute of Technology Research Fund Program for Young Scholars and the Fundamental Research Funds for the Central Universities(No.2018CX01023)
the Beijing-Tianjin-Hebei Basic Research Cooperation Project(No.19JCZDJC64100)
the Beijing Nova Program from Beijing Municipal Science&Technology Commission(No.Z201100006820005)
the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology(2020-2022)。
