87例HIV抗体不确定患者病毒载量检测分析

Analysis of viral load detection in 87 patients with indeterminate HIV-1 antibodies

目的探讨病毒载量(VL)检测对87例艾滋病病毒(HIV)抗体不确定患者感染诊断的价值。方法对蛋白印迹试验(WB)确证为HIV抗体不确定患者进行VL检测及分析,并对这些患者进行随访及流行病学调查,以证实其感染情况。结果87例HIV抗体不确定患者中,VL低于检测限18例(20.69%),VL高于检测限69例(79.31%),其中VL在20~5000拷贝/mL 4例(4.60%),VL>5000拷贝/mL 65例(74.71%)。经随访,VL低于检测限者最终排除了HIV感染,而VL高于检测限者最终诊断为HIV感染。结论VL检测可以快速准确地鉴别HIV抗体不确定患者是否感染,是WB的一种有效补充检测试验方法,有助于提高HIV感染的诊断率。

Objective To evaluate the value of viral load(VL)in diagnosis of 87 patients with indeterminate HIV antibodies.Methods The indeterminate HIV antibody patients confirmed by western blot(WB)were tested with VL,and followed up,then epidemiological investigation were conducted to confirm their infection status.Results Among the 87 patients with indeterminate HIV antibodies,there were 18(20.69%)cases with VL below the detection limit,69(79.31%)cases with VL above the detection limit,4(4.60%)cases with VL between 20-5000 copies/mL,and 65(74.71%)cases with VL above 5000 copies/mL.After follow-up,those with VL lower than the detection limit were finally excluded from HIV infection,while those with VL higher than the detection limit were finally diagnosed as HIV infection.Conclusion VL test can quickly and accurately identify whether the HIV antibody is indeterminate and whether they are infected or not.It is an effective test method to supplement WB,which can improve the diagnosis rate of HIV infection.