木醋液对小麦幼苗生长、抗氧化特性及内源激素含量的影响

Effects of Wood Vinegar on Seedling Growth,Antioxidant Activity and Endogenous Hormone Content of Wheat

研究木醋液对小麦幼苗生长、抗氧化特性以及内源激素含量的影响,探讨木醋液对小麦幼苗生长的调节机制,为木醋液用作植物生长调节剂提供参考。选用不同浓度的杉木木醋液进行小麦幼苗培养实验,以水为对照组(CK),分析不同浓度木醋液对小麦幼苗形态学指标、抗氧化特性以及内源激素含量的影响。研究表明,木醋液浓度通过逆境胁迫效应,改变小麦幼苗抗氧化酶活性和内源激素含量,进而影响其生长。低浓度木醋液培养小麦引起轻微逆境胁迫,小麦幼苗中MDA含量降低而POD和SOD活性增加,同时内源激素IAA和GA3含量增加、ABA含量降低,促进小麦幼苗生长;高浓度木醋液培养小麦引起严重逆境胁迫,MDA含量增加而POD和SOD活性降低,内源激素IAA和GA3含量降低而ABA含量升高,抑制小麦幼苗生长。木醋液对小麦幼苗的生长表现为低浓度促进生长,高浓度抑制生长,浓度为0.25 mL/L时对小麦幼苗生长最有利。

This study investigated the effect of wood vinegar(WV)on seedling growth,antioxidant activity and endogenous hormone content of wheat,and discussed the influence mechanism for the application of WV as growth regulator.The effects of different concentrations of WV on morphological indexes,antioxidant characteristics and endogenous hormone content of wheat seedlings were analyzed by using water as the control group(CK).The results showed that the growth of wheat seedlings was affected by the concentration of WV via the changing of the activity of antioxidant enzymes and the content of endogenous hormones caused by the stress effect of adversity.Low concentration of WV was the slight stress condition to wheat seedling growth,which reduced the MDA content,increased the activities of POD and SOD,and increased the contents of IAA and GA3 and decreased the content of ABA,thus promoted the growth of wheat seedlings.On the contrary,high concentration of WV caused the severe stress on the growth of wheat seedling,which increased the MDA content,decreased the activities of POD and SOD,and reduced the contents of IAA and GA3 and increased the ABA content,resulting in the inhibition of wheat seedling growth.The regulation mechanism of WV on wheat seedling growth could be described as low concentration of WV could promote the wheat seedlings growth and high concentration could inhibit the growth,and the optimal concentration of WV on the growth of wheat seedling is 0.25 mL/L.