建佛手总黄酮的超声波提取工艺优化及抗氧化分析

Flavonoids from Fujian Bergamot:Ultrasonicly Extracting Technology and Its Antioxidation Properties

为研究福建佛手总黄酮的超声波提取技术及其抗氧化性,通过正交实验法确定超声波提取建佛手总黄酮的最优提取工艺,即在超声频率为45 kHz与80 kHz双频模式下,在料液比1:35、乙醇浓度60%、超声功率50 W、提取温度50℃下提取80 min,建佛手总黄酮的提取率最高,可达3.33%。体外抗氧化实验表明,建佛手总黄酮对·OH及O_(2)^(-)·均有明显的清除作用,但对·OH及对O_(2)^(-)·的清除作用均不如同浓度的Vc溶液。当建佛手总黄酮质量浓度为1.4 mg/mL时,佛手总黄酮对·OH和O_(2)^(-)·的清除率分别为54.4%和39.2%。

The paper aims to optimize the extraction process of flavonoids from Fujian bergamot by ultrasonicly method and study its antioxidation activity.The extraction process condition optimized by orthogonal experiments is:under the double frequency of ultrasonic frequency of 45 kHz and 80 kHz,the ratio of material to liquid of 1:35(g:mL),the ethanol concentration of 60%,the ultrasonic power and ultrasonic temperature of 50 W and 50℃respectively,and the extraction time of 80 min,the extraction yield of flavonoids could reach the highest of 3.33%.The scavenging effect of Fujian bergamot flavonoids on·OH and O_(2)^(-)·are obvious and effective,but the antioxidant activity is weaker than that of Vc.When the solubility of Fujian bergamot flavonoids is 1.4 mg/mL,the scavenging rate is 54.4%and 39.2%,respectively.