RRS1对皮肤鳞状细胞癌细胞增殖、转移和侵袭的影响

Effect of RRS1 on the proliferation,metastasis and invasion of cutaneous squamous cell carcinoma cells

目的:明确RRS1(核糖体合成调节因子1)对皮肤鳞状细胞癌(cSCC)细胞增殖、转移和侵袭的影响。方法:通过免疫组化和GEO数据库明确RRS1在cSCC与正常皮肤中表达的差异。慢病毒感染法建立RRS1敲低的cSCC细胞系SCL-1细胞。MTT实验、Celigo划痕实验、Transwell实验研究RRS1敲低后SCL-1细胞增殖、转移和侵袭性的变化。QRT-PCR法检测增殖、转移和侵袭相关基因表达。结果:RRS1在cSCC细胞中表达量明显高于正常皮肤,RRS1敲低后SCL-1细胞增殖、转移和侵袭性均降低。RRS1敲低后,增殖相关基因FGF2,AREG,cyclin E1显著降低。转移和侵袭相关基因VIM,CXCL1,CXCL3,CXCL8,MMP1表达显著降低。结论:RRS1可能参与了cSCC细胞的增殖、转移和侵袭过程。

Objective:To determine the effect of ribosome synthesis regulator 1(RRS1)on the proliferation,metastasis and invasion of cutaneous squamous cell carcinoma(cSCC)cells.Methods:The difference of RRS1 expression in cSCC and normal skin was confirmed by immunohistochemical method and GEO database.SCL-1 cells with RRS1 knockdown were established by lentivirus infection.MTT,Celigo scratch,and Transwell were used to study the proliferation,metastasis and invasiveness of SCL-1 cells after RRS1 knockdown.The expression of genes related to proliferation,metastasis and invasion was detected by QRT-PCR.Results:The expression of RRS1 in cSCC cells was significantly higher than that in normal skin.The proliferation,metastasis and invasiveness of SCL-1 cells were reduced after RRS1 knockdown.After knocking down RRS1,the proliferation-related genes FGF2,AREG,and cyclin E1 were significantly reduced.Metastasis and invasion-related genes VIM,CXCL1,CXCL3,CXCL8,and MMP1 are significantly reduced.Conclusion:RRS1 may participate in the cell proliferation,metastasis and invasion of cSCC.