五维甘草那敏胶囊中泛酸钙、维生素B_(2)含量测定方法改进

Improvement of the Determination of Calcium Pantothenate and Vitamin B_(2) in Pentavitamin,Licorice and Chlorphenamine Maleate Capsules

目的利用高效液相色谱法同时测定五维甘草那敏胶囊中泛酸钙、维生素B_(2)的含量。方法色谱柱:Agilent Eclipse Plus C18(4.6 mm×250 mm,5μm);流动相:甲醇-0.5%磷酸溶液(28∶72);进样量:10μL;柱温为30℃;流速:1.0 m L·min^(-1);检测波长为210 nm。结果泛酸钙、维生素B2分别在40.76~815.2,10.56~211.2μg·m L^(-1)范围内线性关系良好。回归方程分别为:Y_(1)=3.3×10^(3)X+1.4×10^(4)(r=0.999 5,n=6),Y_(2)=1.9×10^(4)X-1.9×10^(3)(r=0.999 4,n=6);平均回收率分别为98.82%(RSD=0.76%)、98.54%(RSD=0.98%)。精密度分别为0.63%、0.88%(n=6)。结论该方法经方法学验证,可用于五维甘草那敏胶囊中泛酸钙、维生素B2的含量测定。

OBJECTIVE To establish a method of simultaneous determination for calcium pantothenate and vitamin B2in pentavitamin,licorice and chlorphenamine maleate capsules by high performance liquid chromatography.METHODS Agilent Eclipse Plus C_(18) column(4.6 mm×250 mm,5μm),with the mobile phase of methanol-0.5%phosphoric acid solution(28∶72) was performed.,The injection volume was 10 uL.The column temperature was set at 30℃.The flow rate was 1.0 mL·min^(-1).The detection wavelength was 210 nm.RESULTS Calcium pantothenate and vitamin B2had a good linear relationship in the range of 40.76-815.2 and 10.56-211.2μg·m L^(-1),respectively.The regression equations were Y_(1)=3.3×10^(3)X+1.4×10^(4)(r=0.999 5,n=6),Y_(2)=1.9×10^(4)X-1.9×10^(3)(r=0.999 4,n=6).The average recovery rates were 98.82%(RSD=0.76%),98.54%(RSD=0.98%).The precision were 0.63%and0.88%,respectively (n=6).CONCLUSION The method is validated by methodologies and can be used for the content determination of calcium pantothenate and vitamin B2in pentavitamin,licorice and chlorphenamine maleate capsules.