摘要
We evaluated a novel and non-destructive method of the electrical impedance spectroscopy(EIS)to elucidate the genetic and evolutionary relationship of homoploid hybrid conifer of Pinus densata(P.d)and its parental species Pinus tabuliformis(P.t)and Pinus yunnanensis(P.y),as well as the artificial hybrids of the P.t and P.y.Field common garden tests of96 trees sampled from 760 seedlings and 480 EIS records of 1,440 needles assessed the interspecific variation of the P.d,P.t,P.y and the artificial hybrids.We found that(1)EIS at different frequencies diverged significantly among germplasms;P.y was the highest,P.t was the lowest,and their artificial hybrids were within the range of P.t and P.y;(2)maternal species effect of EIS magnitudes in the hybrids and P.d was stronger than the paternal species characteristics;(3)EIS of the artificial hybrid confirmed the mid-parent and partial maternal species characteristics;(4)unified exponential model of EIS for the interspecific and hybrids can be constructed as|Z|=Af^(-B);(5)cluster analysis for species and hybrid combinations in total corroborated with the previous hybrid model of Pinus densata.Our non-destructive EIS method complemented the previous finding that Pinus densata was originated from P.t and P.y.We conclude that the impedance would be a viable indicator to investigate the interspecific genetic variations of conifers.
基金
funded by National Natural Research Fund 31070591,“Adaptation research of Pinus densata,Pinus tabuliformis,Pinus yunnanensis and the hybrids at high elevation habitats”,P.R.China.
