摘要
通过比较序列测定法,在NR4A1基因第5内含子发现A/G突变,建立了PCR-DdeI-RFLP分型技术,并在3个中外猪种中进行多态性分型,分析其基因型频率和等位基因频率,还在长白猪猪群中检测该位点的多态性并与产仔数性状进行关联分析。结果表明,在长白猪猪群中,所有胎次产仔数呈现GG>AG>AA的趋势。其中,GG型个体和AG型个体的所有胎次产仔数分别显著高于AA型个体(P<0.05)。因此,NR4A1基因在长白猪猪群中与产仔数性状显著性相关,可能为提高猪产仔数性状提供一个有用的分子标记。
By comparision of the sequences of NR4A1 gene,a A/G mutation in intron 5 was found and developed a PCR-DdeI-RFLP genotyping assay.Genotype frequencyies and allele frequencies of this SNP were investigated in 3 pig breeds.Association of the SNP and litter size was assessed in New Strain Huai PigⅡ.Statistical analysis demonstrated that it showed the tendency of GG>AG>AA in all parity.In addition,those with the GG genotype and AG genotype had additional piglets born compared to the AA animals(P<0.05),respectively in New Strain Huai PigⅡ.Therefore,NR4A1 gene has significant associated with litter size in New Strain Huai PigⅡ,could be a useful molecular marker in selection for increasing litter size in pigs.
作者
刘林清
李庆岗
苏世广
周梅
张威
王重龙
LIU Linqing;LI Qinggang;SU Shiguang;ZHOU Mei;ZHANG Wei;WANG Chonglong(Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences,Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering,Hefei 230031,China)
出处
《养猪》
2021年第2期65-67,共3页
Swine Production
基金
安徽省农业科学院重点实验室项目(2020YL031)
安徽省科技重大专项(17030701008)
国家自然科学基金项目(31371258)
安徽省自然科学基金项目(2008085QC138)
安徽省生猪产业技术体系项目(AHCYTX-05-09)
安徽省生猪遗传改良计划专项(2018FACN4373)。
