回输卡介苗激活的T淋巴细胞重塑裸鼠硬脑膜淋巴管结构

T-Cell Activated by BCG Reinfusion Remodels the Structure of Dural Meningeal Lymphatic Vessels in Nude Mice

【目的】探讨外周免疫对脑膜淋巴管结构的影响。【方法】通过尾静脉给Nude裸鼠回输卡介苗激活的CD3+的T淋巴细胞,建立免疫重构模型小鼠;回输等体积的PBS作为对照组;BALB/c小鼠作为野生型组。采用免疫荧光染色法检测小鼠硬脑膜lyve-1+的淋巴管内皮细胞连续程度对淋巴管结构进行评估,检测硬脑膜淋巴管内CD11b+细胞的数量来反映免疫细胞迁入淋巴管的程度。【结果】免疫荧光染色的结果显示:与BALB/c小鼠相比,裸鼠背侧淋巴管内CD11b+细胞数量降低(P<0.05)。回输T淋巴细胞后裸鼠的脑膜淋巴管较对照组有明显的增殖。裸鼠颈部淋巴结副皮质区淋巴小结增生,皮质区无明显差异。【结论】外周免疫调节可改善脑膜淋巴管的结构及功能。

【Objective】To investigate the effect of peripheral immunity on the structure of meningeal lymphatics.【Methods】BCG activated CD3+T cells were injected back into nude mice through tail vein to establish the immune recon⁃struction model group,while the control group was set up by transfusion of the equal volume of PBS.BALB/c mice were treated as wild-type group.Immunofluorescence staining was used to detect the degree of continuity of Lyve-1 positive lym⁃phoid epithelium in the dural mater of mice to evaluate the structure of lymphatic vessels,and the number of CD11b+cells in the lymphatic vessels was detected to reflect the degree of migration of immune cells into the dural lymphatic vessels.【Results】The immunofluorescence staining results showed that compared with BALB/c mice,the number of CD11b+cells in the dorsal lymphatic vessels of nude mice decreased(P<0.05).Compared with the control group,the dural meningeal lymphatic vessels of nude mice were significantly proliferated after T cell transfusion.There was no significant difference between BALB/c mice and nude mice in the cortical areas of cervical lymph nodes,while lymphoid nodules proliferated in the paracortical areas in nude mice.【Conclusion】Peripheral immune regulation can improve the structure and function of meningeal lymphatic vessels.