白茅苷缓解STZ诱导的Ⅰ型糖尿病幼龄鼠肝损伤

Imperatorin Alleviates the Liver Damage in Young Rats with Streptozotocin-induced Type Ⅰ Diabetes Mellitus

【目的】探讨白茅苷对链脲佐菌素(STZ)诱导的Ⅰ型糖尿病幼龄鼠肝损伤、过氧化应激和免疫应答的影响。【方法】50只BALB/c幼龄鼠分为健康对照组、模型组、模型+3 mg/kg白茅苷组、模型+6 mg/kg白茅苷组、模型+12 mg/kg白茅苷组(n=10)。糖耐量实验检测各组小鼠空腹血糖、血清胰岛素和肥胖指数,自动生化分析仪测定血清中高密度脂蛋白、低密度脂蛋白、甘油三酯、总胆固醇含量,苏木精-伊红染色观察肝损伤程度,末端标记染色观察细胞凋亡情况,试剂盒检测超氧化物歧化酶(SOD)和丙二醛(MDA)含量水平,免疫印迹法检测线粒体中Bax/Bcl-2、cleaved cas3/cas3、c-Myc表达水平,酶联免疫吸附试验(ELISA)检测血清中白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)含量。【结果】与模型组相比,6 mg/kg和12 mg/kg白茅苷治疗后,Ⅰ型糖尿病小鼠空腹血糖[平均差值5.3 mmol/L,95%置信区间(4.5,6.1)mmol/L]、血清胰岛素[平均差值13.2μU/L,95%置信区间(10.6,15.8)μU/L]和肥胖指数[平均差值2.3 kg/m^(2),95%置信区间(2.1,2.5)kg/m^(2)]均降低,血脂四项水平降低,肝损伤得到缓解,肝细胞凋亡降低,SOD含量增加(P<0.05),MDA含量减少(P<0.05),Bax/Bcl-2、cleaved cas3/cas3表达水平下调(P<0.05),c-Myc水平上调(P<0.05),IL-6、TNF-α和IL-1β含量降低(P<0.05)。【结论】白茅苷能够缓解STZ诱导的Ⅰ型糖尿病幼龄鼠肝损伤、过氧化反应和免疫应答。

【Objective】To investigate the effect of imperatorin on liver damage,peroxidative stress and immune re⁃sponse in young rats with type Ⅰ diabetes induced by streptozotocin(STZ).【Methods】Totally 50 BALB/c juvenile mice were equally divided into healthy control group,model group,model plus+3 mg/kg Imperatorin group,model+6 mg/kg Im⁃peratorin group,and model+12 mg/kg Imperatorin group.Glucose tolerance test was used to detect fasting blood glucose,serum insulin and obesity index of mice in each group.Automatic biochemical analyzer was used to determine serum HDL,LDL,TG,TC content.HE staining was used to observe the degree of liver damage.TUNEL staining was used to observe cell apoptosis.SOD and MDA levels were detected by Kit,Box/Bcl-2,cleaved cas3/cas3,c-Myc expression levels in mi⁃tochondria by Western blot,and serum IL-6,TNF-α,IL-1βlevels by ELISA.【Results】Compared with the model group,12 mg/kg Imperatorin significantly reduced fasting blood glucose[mean difference 5.3 mmol/L,95%confidence interval(4.5,6.1)mmol/L],serum insulin[mean difference 13.2μU/L,95%confidence interval(10.6,15.8)μU/L]and obesity index[average difference 2.3 kg/m^(2),95%confidence interval(2.1,2.5)kg/m^(2)]all decreased.The content of SOD increased(P<0.05),while the content of MDA was reduced(P<0.05).The expression level of Bax/Bcl-2,and cleaved cas3/cas3 was down-regulated(P<0.05),while the level of c-Myc was up-regulated(P<0.05),and the content of IL-6,TNF-αand IL-1βwas reduced(P<0.05).【Conclusion】Imperatorin can alleviate the liver damage,peroxidation and im⁃mune response in young rats with STZ-induced type Ⅰ diabetes.