幽门螺杆菌感染胃炎患儿胃黏膜ASICs表达在胃肠道动力中的作用

Expression of acid⁃sensing ion channels in gastric mucosa of patients with Helicobacter pylori infection gastritis and its role in Gastrointestinal motility

目的探讨幽门螺杆菌(Helicobacter pylori,Hp)感染患儿胃黏膜中酸敏感离子通道(acid-sensing ion channels,ASICs)的表达及其在Hp感染胃炎胃肠功能中的作用。方法将22例经内镜诊断的胃炎患者分为幽门螺杆菌阳性组[Hp(+),n=11]和阴性组[Hp(-),n=11]。免疫化学法检测胃炎患儿胃黏膜中ASICs的表达和定位。ELISA法测定血清CCK、SP、GAS和MTL水平。采用Pearson相关性分析ASIC3表达与胃肠道激素的相关性。结果 Hp感染患者胃黏膜ASIC3表达明显升高,血清CCK、SP、MTL水平明显升高。血清CCK、SP、MTL水平与ASIC3表达密切相关。结论 Hp可诱导胃黏膜ASIC3的表达,其影响Hp感染患者的胃肠激素分泌和胃肠功能。

Objective The aim of this study was to investigate the expression of acid⁃sensing ion channels(ASICs)in gastric mucosa of patients infected with Helicobacter pylori(Hp)and its role in the gastrointestinal function of HP infected gastritis.Methods 22 patients with gastritis diagnosed via endoscopy were divided into 2 groups:the Hppositive group(Hp(+),n=11)and the Hp negative group(Hp(-),n=11).The expression and⁃localization of ASICs in gastric mucosa of patients with gastritis were detected by immunochemistry.Serum CCK,SP,GAS and MTL levels were measured with ELISA methods.The correlation between ASICs expression and gastrointestinal hormones was analyzed by Pearson′s analysis.Results The expression of ASIC3 in gastric mucosa and the levels of CCK,SP and MTL in serum were significantly increased in HP infected patients.Moreover the serum levels of CCK,SP and MTL were closely correlated to the expression of ASIC3.Conclusion Hp could induce the expression of ASIC3 in gastric mucosa,which can affect the secretion of gastrointestinal hormones and gastrointestinal function in patients with HP infection.