铜绿假单胞菌lasR/rhlR基因缺陷对小鼠腹腔生物被膜感染的影响

Effect of pseudomonas aeruginosa las R/rhl R mutation on intraperitoneal biofilm infection in mice

目的探讨铜绿假单胞菌(PA)群体感应(QS)系统的lasR/rhlR基因缺陷对小鼠腹腔PA生物被膜(BF感染的影响。方法体外利用无菌引流管切片建立铜绿假单胞菌PAO1野生型和PAO1 lasR/rhlR基因缺陷型菌株生物被膜感染的载体,将两种感染载体和无菌载体分别移植至正常小鼠腹腔构建生物被膜感染模型,术后3d检测PAO1野生型组、PAO1 lasR/rhlR基因缺陷型组、空白对照组的小鼠血常规变化、腹腔感染情况及其细菌学和病理学改变。结果感染3d后,PAO1 lasR/rhlR基因缺陷型组小鼠的腹腔细菌学改变和病理学改变均明显轻于PAO1野生型组。结论lasR/rhlR基因在铜绿假单胞菌生物被膜体内感染中发挥着重要的致病作用。

Objective To understand the pathogenicity of pseudomonas aeruginosa(PA)lasR/rhlR mutation on intraperitoneal biofilm infection in mice.Methods In vitro biofilms of PAO1,PAO1 lasR/rhlR mutants were established by aseptic drainage tube carriers.The drainage tube was used as carrier,which were placed into abdominal cavities of normal mice to establish biofilm infection model.Three days after establishment of the model,the blood routine changes,abdominal cavity infection bacteriological and pathological changes of PAO1 group,PAO1 lasR/rhlR group and control group were observed.Results Three days after infection,the changes of bacteriological and pathological in the PAO1 lasR/rhlR mutants group was significantly lower and milder than PAO1 group.Conclusion lasR/rhlR genes play an important role in the intraperitoneal PA biofilm infection in mice.