HtrA4和VE-cadherin在子痫前期中的表达及意义

Expression and significance of HtrA4 and VE-cadherin in preeclampsia

目的探讨HtrA丝氨酸肽酶4(HtrA4)和血管内皮钙黏素(VE-cadherin)在子痫前期(PE)中的水平变化情况。方法分析该院收治的PE孕妇(根据孕周分为PE早发组和PE晚发组)及健康妊娠晚期孕妇的临床资料,收集母血和胎盘组织,采用酶联免疫吸附测定(ELISA)检测母血和胎盘组织中HtrA4和VE-cadherin水平,采用实时荧光定量PCR(qPCR)和免疫印迹法(Western blot)测定胎盘组织中HtrA4和VE-cadherin的mRNA和蛋白表达水平。结果ELISA结果显示,与健康妊娠晚期孕妇比较,PE孕妇的HtrA4水平升高,PE晚发组HtrA4水平低于PE早发组(P<0.05);PE孕妇的VE-cadherin水平降低,PE晚发组VE-cadherin水平高于早发组(P<0.05)。qPCR和Western blot结果显示,与健康妊娠晚期孕妇比较,HtrA4和VE-cadherin的mRNA和蛋白表达水平在PE孕妇中降低(P<0.05);PE早发组HtrA4的mRNA和蛋白表达水平低于晚发组,PE早发组VE-cadherin的mRNA和蛋白表达水平高于晚发组(P<0.05)。结论HtrA4和VE-cadherin均参与PE的发生发展,VE-cadherin的表达可能与PE滋养细胞的侵袭分化有关。

Objective To investigate the level changes of HtrA serine peptidase 4(HtrA4)and vascular endothelial cadherin(VE-cadherin)in preeclampsia(PE).Methods The clinical data of PE pregnant women(divided into early PE group and late PE group according to gestational age)and healthy pregnant women in late pregnancy were analyzed.The levels of HtrA4 and VE-cadherin in maternal blood and placenta were detected by enzyme-linked immunosorbent assay(ELISA).The mRNA and protein expression levels of HtrA4 and VE-cadherin in placenta were determined by real-time fluorescent quantitative PCR(qPCR)and Western blot.Results The results of ELISA showed that the level of HtrA4 in PE pregnant women was higher than that in healthy pregnant women in late pregnancy,the level of HtrA4 in late PE group was lower than that in early PE group,the level of VE-cadherin in PE pregnant women was lower than that in healthy pregnant women in late pregnancy,and the level of VE-cadherin in late PE group was higher than that in early PE group(all P<0.05).The results of qPCR and Western blot showed that the mRNA and protein expression levels of HtrA4 and VE-cadherin in PE pregnant women were lower than those in healthy pregnant women(P<0.05).The mRNA and protein expression levels of HtrA4 in early PE group were lower than those in late PE group,and the mRNA and protein expression levels of VE-cadherin in early PE group were higher than those in late PE group(P<0.05).Conclusion HtrA4 and VE-cadherin are involved in the occurrence and development of PE.The expression of VE cadherin may be related to the invasion and differentiation of PE trophoblast.