地塞米松对组织细胞辐射旁效应及其再传递的拮抗

Antagonism of dexamethasone to radiation bystander effect and its re-transmission

目的:观察地塞米松对组织细胞辐射旁效应及其再传递的拮抗作用。方法:使用6MV-X射线照射鼠卵巢癌NUTU19细胞株,其培养液制备第1代条件培养液,处理第1代效应细胞鼠源NUTU19细胞株、肠黏膜上皮IEC-6细胞株,由第1代鼠源效应细胞的分泌物为第2代条件培养液,再处理第2代效应细胞NUTU19细胞株、IEC-6细胞株和鼠淋巴细胞,检测细胞的生长活力、凋亡水平,各实验组均增加使用地塞米松作为平行处理对照。结果:地塞米松对第1代、第2代条件培养液处理的鼠卵巢癌细胞株的增殖活力没有显示出保护作用;对肠黏膜上皮细胞株的增殖活力保护明显,第1代达到23%,第2代达到33%。第1代、第2代条件培养液对鼠淋巴细胞没有显示出特别的损伤作用,地塞米松也没有显示出提高细胞活力的作用。地塞米松干预后,第1代、第2代条件培养液处理的鼠卵巢癌细胞株的凋亡水平分别减低5.8%和4.0%,肠黏膜上皮细胞减低6%和3%。鼠淋巴细胞未显示条件培养液的杀伤作用和地塞米松的保护作用。结论:在一定的条件下,地塞米松能够拮抗肠上皮细胞、淋巴细胞辐射旁效应的损伤,并可以拮抗旁效应的再传递。

Objective:To observe the antagonism of dexamethasone on radiation bystander effect and its re-transmission.Methods:6MV X-ray irradiation of mouse ovarian cancer cell line NUTU19 was used to prepare the first generation conditioned medium.The first-generation effecter cell line NUTU19 and intestinal mucosal epithelial IEC-6 were treated with the second generation conditioned medium from the secretion of the first-generation effecter cells.The second-generation effecter cell line NUTU19,IEC-6 and mice lymphocyte were treated with the second generation conditioned medium.The cell growth activity and apoptotic level were detected.Dexamethasone was used as parallel treatment control in all experimental groups.Results:There was no protective effect on NUTU19 cell lines treated with conditioned medium of the first and second generation conditioned medium,and the intestinal mucosal epithelial IEC-6 cell lines was significantly protected,reaching 23%in the first generation conditioned medium and 33%in the second generation conditioned medium.The conditioned medium of the first and second generation did not show any special damage to the lymphocyte of mice,and dexamethasone did not show the effect of increasing cell viability.After the intervention of dexamethasone,the apoptotic level of ovarian cancer cell lines treated with conditioned medium of the first and second generations decreased by 5.8%and 4.0%,and the epithelial cells of intestinal mucosa decreased by 6%and 3%.The killing effect of conditioned medium and the protective effect of dexamethasone were not observed in mouse lymphocytes.Conclusion:Under certain conditions,dexamethasone can antagonize the injury of intestinal epithelial cells and lymphocyte by radiation bystander effect,and can also antagonize the re-transmission of radiation bystander effect.