IGFBP2增强IGF-1诱导的HepG2细胞增殖、迁移和侵袭

IGFBP2 enhances IGF-1 induced proliferation,migration and invasion of HepG2 cells

目的探讨胰岛素样生长因子结合蛋白2(IGFBP2)对胰岛素样生长因子-1(IGF-1)诱导人源肝癌(HCC)细胞株HepG2细胞增殖、迁移和侵袭能力的影响及其机制。方法选用15.6、31.2、62.5、125、250 ng/ml的IGFBP2与50 ng/ml的IGF-1联合刺激HepG2细胞。用CCK-8法检测细胞增殖,transwell法检测细胞迁移和侵袭;Western blot检测细胞内信号通路相关蛋白的活化和表达。结果与IGF-1单独刺激相比,IGFBP2与IGF-1联合作用可增强HepG2细胞增殖、迁移和侵袭能力;IGFBP2与IGF-1联合刺激增加HepG2细胞株中IGF1R、Akt和ERK磷酸化水平,而对总表达无明显改变,同时上调早期生长反应蛋白1(EGR1)的表达。结论IGFBP2通过活化Akt及ERK信号通路增强IGF-1诱导HepG2细胞增殖、迁移和侵袭的能力。

Objective To investigate the effect and mechanism of insulin-like growth factor binding protein 2(IGFBP2)on insulin-like growth factor-1(IGF-1)induced proliferation,migration and invasion of hepatocellular carcinoma(HCC)cell line of HepG2 cells.Methods 15.6,31.2,62.5,125,250 ng/ml IGFBP2 and 50 ng/ml IGF-1 were used to stimulate HepG2 cells.Cell proliferation was detected by CCK-8 method,and cell migration and invasion were detected by transwell method.Western blot was used to detect the activation and expression of intracellular signaling pathway-related proteins.Results The combination of IGFBP2 and IGF-1 could promote the proliferation,migration and invasion of HepG2 cells compared with the effect of IGF-1.The combination of IGFBP2 and IGF-1 increased phosphorylation levels of IGF1R,Akt and ERK in HepG2 cells without significant changes in total expression,and the protein expression of early growth response gene 1(EGR1)also increased.Conclusion IGFBP2 enhances IGF-1 induced proliferation,migration and invasion of HepG2 cells by activating Akt and ERK signaling pathways.